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| [[Image:3gpt.jpg|left|200px|thumb|Crystal Structure of Proteasome, [[3gpt]]]]
| | <StructureSection load='' size='350' side='right' scene='41/417543/Cv/1' caption='Yeast proteasome 20S core complex with salinosporamide derivative, [[3gpt]]'> |
| {{STRUCTURE_3gpt| PDB=3gpt | SIZE=300| SCENE= |right|CAPTION=Proteasome, [[3gpt]] }}
| | == Function == |
| | [[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides<ref>PMID:22350895</ref>. The '''26S PRTS''' is composed of a central '''20S core particle''' which contains 4 stacked rings each with several members and two 19S caps. The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which bind polyubiquitin. <br /> |
| | *'''13S PRTS''' is a PRTS core intermediate containing α ring, partial β ring and 3 chaperones<ref>PMID:33846632</ref>. |
| | *'''15S PRTS''' is half of an 20S PRTS and upon dimerisation forms the mature 20S PRTS<ref>PMID:32442437</ref>. |
| | For more details see [[3unb]]. |
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| | | == Structural highlights == |
| [[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides. The 26S PRTS is composed of a central 20S core particle which contains 4 stacked rings each with several members and two 19S caps. The core particle two outer rings contain 7 alpha subunits which form the PRTS gate. The two inner rings contain 6 beta subunits with protease activity. The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which binds polyubiquitin. The images at the left and at the right correspond to one representative Proteasome, ''i.e.'' the crystal structure of yeast Proteasome ([[3gpt]]).
| | The core particle two outer rings contain 7 α subunits (Y7, Y13, PRE6, PRE5, PUP2, C1, C7-α) which form the PRTS gate. The two inner rings contain 7 β subunits (PRE2, PRE4, PRE3, PUP1, PUP3, C5, C11) with protease activity<ref>PMID:8811196</ref>. |
| | | *<scene name='41/417543/Cv/2'>α subunits and β subunits</scene>. |
| {{TOC limit|limit=2}}
| | *<scene name='41/417543/Cv/6'>Salinosporamide derivative binding site</scene> of chain H. |
| | *<scene name='41/417543/Cv/7'>Covalent bond between Salinosporamide derivative and Thr1</scene>. |
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| == 3D Structures of Proteasome == | | == 3D Structures of Proteasome == |
| | [[Proteasome 3D structures]] |
| | == See Also == |
| | [[PROTAC]] (Proteolysis-Targeting Chimera), an alternative to conventional enzyme inhibitors, by enabling proteasome induced degradation of the target protein. |
| | </StructureSection> |
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| ''Update June 2011''
| | == References == |
| | | <references/> |
| === 20S particle ===
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| [[3mfe]], [[3mi0]], [[2fhg]] – Mt20S core – ''Mycobacterium tuberculosis''<br />
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| [[3gpt]], [[3gpw]], [[1g0u]] – y20S core - yeast<br />
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| [[1ryp]] - y20S core (mutant)<br />
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| [[2zcy]] – y20S+syringolin<br />
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| [[3mka]], [[3hfa]] – Mt20S core (mutant)<br />
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| [[3c91]], [[3c92]], [[1pma]] – Ta20S core - ''Themoplasma acidophilum''<br />
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| [[3h4p]] – 20S core – ''Methanocaldococcus janaschii''<br />
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| [[2h6j]], [[1q5r]] – Re20S core (mutant) – ''Rhodococcus erythropolis''<br />
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| [[1q5q]] - Re20S core<br />
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| [[1j2q]] – Af20S core - ''Archaeoglobus fulgidus''<br />
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| [[1iru]] – 20S core - bovine<br />
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| === 20S+activator protein ===
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| [[1vsy]], [[1l5q]] – y20S core+ Blm10 <br />
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| [[1z7q]], [[1fnt]] – y20S core+PA26<br />
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| [[3ipm]], [[3jrm]], [[3jse]], [[3jtl]], [[1ya7]], [[1yar]], [[1yau]] - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U <br />
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| === 20S+inhibitor ===
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| [[3hye]], [[3gpj]], [[3dy3]], [[3dy4]], [[3e47]], [[3d29]], [[3bdm]], [[2zcy]], [[2gpl]], [[2fak]], [[2f16]], [[1jd2]], [[1g65]], [[3mg0]], [[3mg4]], [[3mg6]], [[3mg7]], [[3mg8]] – y20S core+inhibitor<br />
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| [[3h6f]], [[3h6i]], [[3hf9]], [[2fhh]], [[3krd]] – Mt20S core+inhibitor<br />
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| [[3nzj]], [[3nzw]], [[3nzx]] - y20S + TMC-95A mimic ligand
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| === Partial 20S ===
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| [[2ku1]], [[2ku2]] – Taa-PRTS chains A-G <br />
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| [[3fp9]] – MtATPase chains A-L<br />
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| [[1j2p]] – Af α subunit<br />
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| [[2jay]] – Mt β subunit<br />
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| [[2kqz]], [[2kr0]] – hPRTS ubiquitin receptor domain
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| ===Partial 26S===
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| [[1p9c]] – hPRTS subunit 4 C-terminal – human<br />
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| [[1p9d]] - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain<br />
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| [[1uel]] - hPRTS subunit 4 C-terminal + HHR23A ubiquitin-like domain - NMR<br />
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| [[1yx4]] - hPRTS subunit 4 – NMR<br />
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| [[1yx5]], [[1yx6]], [[2kde]], [[2kdf]] - hPRTS subunit 4+ubiquitin – NMR<br />
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| [[2o95]], [[2o96]] - hPRTS subunit 7<br />
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| [[3kw6]] - hPRTS subunit 8<br />
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| [[2krk]] - hPRTS subunit 8 – NMR<br />
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| [[1qym]], [[1uoh]] - hPRTS subunit 10<br />
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| [[1tr4]] - hPRTS subunit 10 – NMR<br />
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| [[2dvw]], [[2dwz]] - mPRTS subunit 10 + hPRTS subunit 6B – mouse<br />
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| [[3aji]] - mPRTS subunit 10 + subunit 4<br />
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| [[2x5n]] - PRTS subunit RPN10 VWA domain – fission yeast<br />
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| [[2z4d]] - yPRTS subunit RPN13<br />
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| [[2z59]] - mPRTS subunit RPN13+ubiquitin
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| [[Category:Topic Page]] | | [[Category:Topic Page]] |