3o0a: Difference between revisions

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-{{Seed}}
-[[Image:3o0a.jpg|left|200px]]
-<!--
+==Crystal structure of the wild type CP1 hydrolitic domain from Aquifex Aeolicus leucyl-trna==
-The line below this paragraph, containing "STRUCTURE_3o0a", creates the "Structure Box" on the page.
+<StructureSection load='3o0a' size='340' side='right'caption='[[3o0a]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3o0a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O0A FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o0a OCA], [https://pdbe.org/3o0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o0a RCSB], [https://www.ebi.ac.uk/pdbsum/3o0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o0a ProSAT]</span></td></tr>
-{{STRUCTURE_3o0a|  PDB=3o0a  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SYLA_AQUAE SYLA_AQUAE]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The editing or hydrolytic CP1 domain of leucyl-tRNA synthetase (LeuRS) hydrolyses several misactivated amino acids. The CP1 domain of Aquifex aeolicus LeuRS was expressed, purified and crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.8, b = 98.4, c = 116.7 A. Crystals diffract to beyond 1.8 A resolution and contain two monomers in the asymmetric unit. Two CP1 mutants in which a conserved threonine residue essential for the fidelity of the hydrolytic pathway is mutated to alanine or glutamic acid have also been expressed and crystallized. Crystals of the two CP1 mutants are isomorphs of the wild type and diffract to beyond 1.9 A resolution. All structures were solved by molecular-replacement techniques.
-===Crystal structure of the wild type CP1 hydrolitic domain from Aquifex Aeolicus leucyl-trna===
+Crystallization and preliminary X-ray crystallographic study of the wild type and two mutants of the CP1 hydrolytic domain from Aquifex aeolicus leucyl-tRNA synthetase.,Cura V, Olieric N, Guichard A, Wang ED, Moras D, Eriani G, Cavarelli J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt, 10):899-901. Epub 2005 Sep 13. PMID:016511190<ref>PMID:016511190</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3o0a" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-O0A is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0A OCA].
+*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:16511190</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Aquifex aeolicus]]
-[[Category: Leucine--tRNA ligase]]
+[[Category: Large Structures]]
-[[Category: Cavarelli, J.]]
+[[Category: Cavarelli J]]
-[[Category: Cura, V.]]
+[[Category: Cura V]]
-[[Category: Eriani, G.]]
+[[Category: Eriani G]]
-[[Category: Moras, D.]]
+[[Category: Moras D]]
-[[Category: Olieric, N.]]
+[[Category: Olieric N]]
-[[Category: Wang, E D.]]
+[[Category: Wang E-D]]
-[[Category: Aminoacyl-trna synthetase]]
-[[Category: Aquifex aeolicus]]
-[[Category: Atp-binding]]
-[[Category: Cp1 hydrolytic domain]]
-[[Category: Leucyl-trna synthetase]]
-[[Category: Ligase]]
-[[Category: Nucleotide-binding]]
-[[Category: Protein biosynthesis]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Nov 18 00:51:47 2010''