3al2: Difference between revisions

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-[[Image:3al2.png|left|200px]]
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+==Crystal Structure of TopBP1 BRCT7/8==
-The line below this paragraph, containing "STRUCTURE_3al2", creates the "Structure Box" on the page.
+<StructureSection load='3al2' size='340' side='right'caption='[[3al2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3al2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AL2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_3al2|  PDB=3al2  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3al2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3al2 OCA], [https://pdbe.org/3al2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3al2 RCSB], [https://www.ebi.ac.uk/pdbsum/3al2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3al2 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The diverse roles of TopBP1 in DNA replication and checkpoint signaling are associated with the scaffolding ability of TopBP1 to initiate various protein-protein interactions. The recognition of the BACH1/FANCJ helicase by TopBP1 is critical for the activation of the DNA replication checkpoint at stalled replication forks and is facilitated by the C-terminal tandem BRCT7/8 domains of TopBP1 and a phosphorylated Thr(1133) binding motif in BACH1. Here we provide the structural basis for this interaction through analysis of the x-ray crystal structures of TopBP1 BRCT7/8 both free and in complex with a BACH1 phospho-peptide. In contrast to canonical BRCT-phospho-peptide recognition, TopBP1 BRCT7/8 undergoes a dramatic conformational change upon BACH1 binding such that the two BRCT repeats pivot about the central BRCT-BRCT interface to provide an extensive and deep peptide-binding cleft. Additionally, we provide the first structural mechanism for Thr(P) recognition among BRCT domains. Together with systematic mutagenesis studies, we highlight the role of key contacts in governing the unique specificity of the TopBP1-BACH1 interaction.
-===Crystal Structure of TopBP1 BRCT7/8===
+Molecular basis of BACH1/FANCJ recognition by TopBP1 in DNA replication checkpoint control.,Leung CC, Gong Z, Chen J, Glover JN J Biol Chem. 2011 Feb 11;286(6):4292-301. Epub 2010 Dec 2. PMID:21127055<ref>PMID:21127055</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3al2" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21127055}}, adds the Publication Abstract to the page
+*[[Topoisomerase binding protein|Topoisomerase binding protein]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21127055 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21127055}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3al2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AL2 OCA].
-==Reference==
-<ref group="xtra">PMID:021127055</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
-[[Category: Glover, J N.]]
+[[Category: Large Structures]]
-[[Category: Leung, C C.]]
+[[Category: Glover JN]]
-[[Category: Brct domain]]
+[[Category: Leung CC]]
-[[Category: Dna binding protein]]
-[[Category: Protein binding]]