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| == Relevance == | | == Relevance == |
| Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. | | Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. '''NanoLuc luciferase''' is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>. |
| </StructureSection> | |
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| == 3D Structures of Luciferase == | | == 3D Structures of Luciferase == |
| | [[Luciferase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| * Luciferase
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| **[[1ba3]] – fLuc+bromoform - firefly<br />
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| **[[3qya]] – LtLuc (mutant) – ''Lampyris turkestanicus''<br />
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| **[[4m46]] – LtLuc<br />
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| **[[3fgc]] – VhLuc α+β chain+FMN – ''Vibrio harveyi''<br />
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| **[[1luc]], [[1brl]] - VhLuc α+β chain<br />
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| **[[1bsl]], [[1xkj]] - VhLuc β chain<br />
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| *Luciferin 2-monooxygenase
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| **[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
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| **[[5b0u]] – Luc2mo (mutant) catalytic subunit – luminous shrimp<br />
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| *Luciferin 4-monooxygenase
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| **[[3iep]], [[1lci]], [[5dv9]], [[3ier]] – fLuc <br />
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| **[[5kyt]], [[5kyv]] – fLuc (mutant)<br />
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| **[[5dwv]] – fLuc+ substrate analog<br />
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| **[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br />
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| **[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br />
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| **[[4g37]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br />
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| **[[5gz2]] – fLuc + thiazole derivative<br />
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| **[[5gyz]] – fLuc + AMP + thiazole derivative<br />
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| **[[5wys]] – fLuc + inhibitor<br />
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| **[[2d1q]] – LcLuc (mutant)+MgATP – ''Luciola cruciata''<br />
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| **[[2d1r]] - LcLuc (mutant)+oxyluciferin+AMP<br />
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| **[[2d1s]], [[2d1t]] - LcLuc (mutant)+intermediate analog<br />
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| **[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
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| }}
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| ==Additional information== | | ==Additional information== |
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| Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms. | | Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms. |
| {{Clear}} | | {{Clear}} |
| *[[Luciola cruciata luciferase]] (Japanese firefly), complexed with Oxyluciferin & AMP ([[2d1r]]) or with an high-energy analogue ([[2d1s]])
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| *[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>
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| *[[1ba3]] firefly luciferase enzyme from ''Photinus pyralis''
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| *[[2psd]], [[2pse]], [[2psf]], [[2psh]], [[2psj]], and [[2rh7]] the luciferase from ''Renilla reniformis'' (RLuc)
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| *[[1bsl]] 1.95Å heterodimer of bacterial luciferase from ''Vibrio harveyi''
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| *[[1brl]] 2.4Å heterodimer of bacterial luciferase from ''Vibrio harveyi''
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| *[[1xkj]] homodimer of bacterial luciferase from ''Vibrio harveyi''
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| *[[2d1q]], [[2d1r]] and [[2d1s]] thermostable Japanese Firefly (''Luciola cruciata'') Luciferase complexed with High-energy intermediate analog
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| *[[2d1t]] thermostable Japanese Firefly Luciferase red-color emission S286N mutant
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| *[[1lci]] firefly luciferase enzyme from ''Photinus pyralis''
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| *[[Dinoflagellate luciferase]]
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| *[[1luc]] The 1.5Å resolution crystal structure of bacterial luciferase from ''Vibrio harveyi''
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| *[[3fgc]] Bacterial luciferase from ''Vibrio harveyi''<ref>PMID: 19435287</ref>
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| *[[1vpr]] luciferase of ''Lingulodinium polyedrum'', a marine bioluminescent dinoflagellate
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| *[[Green Fluorescent Protein]]
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| * For additional information, see: [[Colored & Bioluminescent Proteins]] | | * For additional information, see: [[Colored & Bioluminescent Proteins]] |
| </StructureSection>
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| ==References== | | ==References== |
| {{Reflist}} | | {{Reflist}} |