Luciferase: Difference between revisions

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<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
 
== Function ==
[[Luciferase]] (Luc) is an oxidative enzyme used in bioluminescence.  It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light.  See detailed analysis in<br />
[[Luciferase]] (Luc) or '''luciferin monooxygenase''' (Lucmo)is an oxidative enzyme used in bioluminescence.  It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>.  See detailed analysis in<br />
* [[Luciola cruciata luciferase]]<br />
* [[Luciola cruciata luciferase]]<br />
* [[Luciferase FMN complex- Vibrio harveyi]]<br />
* [[Luciferase FMN complex- Vibrio harveyi]]<br />
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See [[Colored & Bioluminescent Protein]].
See [[Colored & Bioluminescent Protein]].
== Relevance ==
Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>.  '''NanoLuc luciferase''' is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>.


== 3D Structures of Luciferase ==
== 3D Structures of Luciferase ==
[[Luciferase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
 
[[3iep]], [[1lci]] – fLuc – firefly<br />
[[3ier]] – fLuc+PEG 400<br />
[[1ba3]] – fLuc+bromoform<br />
[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br />
[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br />
[[4g37]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br />
[[2d1q]] – LcLuc (mutant)+MgATP – ''Luciola cruciata''<br />
[[2d1r]] - LcLuc (mutant)+oxyluciferin+AMP<br />
[[2d1s]], [[2d1t]] - LcLuc (mutant)+intermediate analog<br />
[[3qya]] – LtLuc (mutant) – ''Lampyris turkestanicus''<br />
[[4m46]] – LtLuc<br />
[[3fgc]] – VhLuc α+β chain+FMN – ''Vibrio harveyi''<br />
[[1luc]], [[1brl]] - VhLuc α+β chain<br />
[[1bsl]], [[1xkj]] - VhLuc β chain<br />
[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
 
 


==Additional information==
==Additional information==
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Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
{{Clear}}
{{Clear}}
*[[Luciola cruciata luciferase]] (Japanese firefly), complexed with Oxyluciferin & AMP ([[2d1r]]) or with an high-energy analogue ([[2d1s]])
 
*[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>
*[[1ba3]] firefly luciferase enzyme from ''Photinus pyralis''
*[[2psd]], [[2pse]], [[2psf]], [[2psh]], [[2psj]], and [[2rh7]] the luciferase from ''Renilla reniformis'' (RLuc)
*[[1bsl]] 1.95&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
*[[1brl]] 2.4&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
*[[1xkj]] homodimer of bacterial luciferase from ''Vibrio harveyi''
*[[2d1q]], [[2d1r]] and [[2d1s]] thermostable Japanese Firefly (''Luciola cruciata'') Luciferase complexed with High-energy intermediate analog
*[[2d1t]] thermostable Japanese Firefly Luciferase red-color emission S286N mutant
*[[1lci]] firefly luciferase enzyme from ''Photinus pyralis''
*[[Dinoflagellate luciferase]]
*[[1luc]] The 1.5&Aring; resolution crystal structure of bacterial luciferase from ''Vibrio harveyi''
*[[3fgc]] Bacterial luciferase from ''Vibrio harveyi''<ref>PMID: 19435287</ref>
*[[1vpr]] luciferase of ''Lingulodinium polyedrum'', a marine bioluminescent dinoflagellate
*[[Green Fluorescent Protein]]
* For additional information, see: [[Colored & Bioluminescent Proteins]]
* For additional information, see: [[Colored & Bioluminescent Proteins]]
</StructureSection>
 
==References==
==References==
{{Reflist}}
{{Reflist}}

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Wayne Decatur, David Canner, Loïc Gazquez, Alexander Berchansky, Michal Harel, Charlene Planchenault
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