Luciferase: Difference between revisions

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Wayne Decatur, David Canner, Loïc Gazquez, Alexander Berchansky, Michal Harel, Charlene Planchenault

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-==See Also==
+<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
-*[[Dinoflagellate luciferase]]
+== Function ==
-*[[Luciola cruciata luciferase]]
+[[Luciferase]] (Luc) or '''luciferin monooxygenase''' (Lucmo)is an oxidative enzyme used in bioluminescence.  It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>.  See detailed analysis in<br />
-*[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>
+* [[Luciola cruciata luciferase]]<br />
-*[[1ba3]] firefly luciferase enzyme from ''Photinus pyralis''
+* [[Luciferase FMN complex- Vibrio harveyi]]<br />
-*[[2psd]], [[2pse]], [[2psf]], [[2psh]], [[2psj]], and [[2rh7]] the luciferase from ''Renilla reniformis'' (RLuc)
+* [[Dinoflagellate luciferase]]<br />
-*[[1bsl]] 1.95&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
+* [[Luciferase (Hebrew)]].
-*[[1brl]] 2.4&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
-*[[1xkj]] homodimer of bacterial luciferase from ''Vibrio harveyi''
+See [[Colored & Bioluminescent Protein]].
-*[[2d1q]], [[2d1r]] and [[2d1s]] thermostable Japanese Firefly (''Luciola cruciata'') Luciferase complexed with High-energy intermediate analog
-*[[2d1t]] thermostable Japanese Firefly Luciferase red-color emission S286N mutant
+== Relevance ==
-*[[1lci]] firefly luciferase enzyme from ''Photinus pyralis''
+Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>.  '''NanoLuc luciferase''' is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>.
-*[[1luc]] The 1.5&Aring; resolution crystal structure of bacterial luciferase from ''Vibrio harveyi''
-*[[3fgc]] Bacterial luciferase from ''Vibrio harveyi''<ref>PMID: 19435287</ref>
+== 3D Structures of Luciferase ==
-*[[1vpr]] luciferase of ''Lingulodinium polyedrum'', a marine bioluminescent dinoflagellate
+[[Luciferase 3D structures]]
-*[[Green Fluorescent Protein]]
+</StructureSection>
+==Additional information==
+[[Image:2d1s jena3dviewer image.jpg|left|220px]]
+<scene name='Luciferase/2d1s/1'>Luciferase</scene> ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}
+Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
+{{Clear}}
+* For additional information, see: [[Colored & Bioluminescent Proteins]]
 ==References==
 {{Reflist}}
-==Additional Literature and Resources==
+==Additional Literature==
 <ref group="xtra">PMID: 18949818</ref><ref group="xtra">PMID: 19859663</ref><references group="xtra"/>
+==External Resources==
+* [http://news.yahoo.com/s/ap/20100911/ap_on_re_us/us_fireflies_military_research Yahoo news coverage of luciferase studies funded research for military applications]
 * [http://en.wikipedia.org/wiki/Luciferase Luciferase at Wikipedia]
 * [http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase: June 2006 Molecule of the Month] as part of the series of tutorials that are at [http://www.pdb.org/pdb/home/home.do the RCSB Protein Data Bank] and written by [[User:David_S._Goodsell|David S. Goodsell]]
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 [[Category: Photoprotein]]
 [[Category: Alpha/beta-hydrolase]]
+[[Category:Topic Page]]