3nmn: Difference between revisions

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-{{Seed}}
-[[Image:3nmn.png|left|200px]]
-<!--
+==Crystal structure of pyrabactin-bound abscisic acid receptor PYL1 in complex with type 2C protein phosphatase ABI1==
-The line below this paragraph, containing "STRUCTURE_3nmn", creates the "Structure Box" on the page.
+<StructureSection load='3nmn' size='340' side='right'caption='[[3nmn]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3nmn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NMN FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PYV:4-BROMO-N-(PYRIDIN-2-YLMETHYL)NAPHTHALENE-1-SULFONAMIDE'>PYV</scene></td></tr>
-{{STRUCTURE_3nmn|  PDB=3nmn  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nmn OCA], [https://pdbe.org/3nmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nmn RCSB], [https://www.ebi.ac.uk/pdbsum/3nmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nmn ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PYL1_ARATH PYL1_ARATH] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA.<ref>PMID:19407143</ref> <ref>PMID:19898420</ref> <ref>PMID:19855379</ref> <ref>PMID:19893533</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/3nmn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nmn ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The phytohormone abscisic acid (ABA) functions through a family of fourteen PYR/PYL receptors, which were identified by resistance to pyrabactin, a synthetic inhibitor of seed germination. ABA activates these receptors to inhibit type 2C protein phosphatases, such as ABI1, yet it remains unclear whether these receptors can be antagonized. Here we demonstrate that pyrabactin is an agonist of PYR1 and PYL1 but is unexpectedly an antagonist of PYL2. Crystal structures of the PYL2-pyrabactin and PYL1-pyrabactin-ABI1 complexes reveal the mechanism responsible for receptor-selective activation and inhibition, which enables us to design mutations that convert PYL1 to a pyrabactin-inhibited receptor and PYL2 to a pyrabactin-activated receptor and to identify new pyrabactin-based ABA receptor agonists. Together, our results establish a new concept of ABA receptor antagonism, illustrate its underlying mechanisms and provide a rational framework for discovering novel ABA receptor ligands.
-===Crystal structure of pyrabactin-bound abscisic acid receptor PYL1 in complex with type 2C protein phosphatase ABI1===
+Identification and mechanism of ABA receptor antagonism.,Melcher K, Xu Y, Ng LM, Zhou XE, Soon FF, Chinnusamy V, Suino-Powell KM, Kovach A, Tham FS, Cutler SR, Li J, Yong EL, Zhu JK, Xu HE Nat Struct Mol Biol. 2010 Sep;17(9):1102-8. Epub 2010 Aug 22. PMID:20729862<ref>PMID:20729862</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3nmn" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20729862}}, adds the Publication Abstract to the page
+*[[Abscisic acid receptor 3D structures|Abscisic acid receptor 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20729862 is the PubMed ID number.
+*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
--->
+== References ==
-{{ABSTRACT_PUBMED_20729862}}
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-NMN is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NMN OCA].
-==Reference==
-<ref group="xtra">PMID:20729862</ref><references group="xtra"/>
 [[Category: Arabidopsis thaliana]]
-[[Category: Phosphoprotein phosphatase]]
+[[Category: Large Structures]]
-[[Category: Kovach, A.]]
+[[Category: Kovach A]]
-[[Category: Li, J.]]
+[[Category: Li J]]
-[[Category: Melcher, K.]]
+[[Category: Melcher K]]
-[[Category: Ng, L M.]]
+[[Category: Ng L-M]]
-[[Category: Soon, F F.]]
+[[Category: Soon F-F]]
-[[Category: Suino-Powell, K M.]]
+[[Category: Suino-Powell KM]]
-[[Category: Xu, H E.]]
+[[Category: Xu HE]]
-[[Category: Xu, Y.]]
+[[Category: Xu Y]]
-[[Category: Yong, E L.]]
+[[Category: Yong E-L]]
-[[Category: Zhou, X E.]]
+[[Category: Zhou XE]]
-[[Category: Abscisic acid signaling]]
-[[Category: Plant hormone receptor]]
-[[Category: Protein binding]]
-[[Category: Pyl1]]
-[[Category: Pyrabactin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 15 10:25:53 2010''