Laccase: Difference between revisions
Michal Harel (talk | contribs) No edit summary |
Michal Harel (talk | contribs) No edit summary |
||
| (16 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
< | <StructureSection load='1w6l' size='350' side='right' scene='' caption='CotA laccase complex with glycerol, O2 and Cu+2 (orange), [[1w6l]]'> | ||
== Function == | |||
''' | '''Laccase''' (Lac) or '''multicopper oxidase''' is a multi-copper protein which uses molecular oxygen to oxidize various aromatic and non-aromatic compounds by a radical-catalyzed reaction mechanism<ref>PMID:15036303</ref>. The multi-copper oxidases constitute a family of enzymes whose principal members are laccase (benzenediol oxygen oxidoreductase, EC 1.10.3.2), ascorbate oxidase (L-ascorbate oxygen oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen oxidoreductase, EC 1.16.3.1). | ||
The multi-copper oxidases constitute a family of enzymes whose | |||
principal members are laccase (benzenediol oxygen oxidoreductase, | |||
EC 1.10.3.2), ascorbate oxidase (L-ascorbate oxygen | |||
oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen | |||
oxidoreductase, EC 1.16.3.1). | |||
* '''Laccase 2''' (Lac2) acts in lignin degradation and in detoxification of lignin products. Typically, laccases show a three cupredoxin-domain folding<ref>PMID:25586560</ref>. | |||
*'''Two-domain laccase''' or small lactase have unusual resistance to inhibitors<ref>PMID:25778839</ref>. | |||
*'''CotA laccase''' belongs to the multi-copper oxidase family. Similar to the other laccases the three dimensional structure of CotA [[1w6l]] comprises three cupredoxin domains and four copper ions organised in <scene name='CotA_laccase/Copper_centers/5'>Two copper centers</scene>: | |||
a <scene name='40/404916/Cv/3'>mononuclear blue type 1 copper center</scene> and <scene name='40/404916/Cv/2'>a trinuclear center</scene>.<ref>PMID:11514528</ref><ref>PMID:16234932</ref> | |||
For laccase with nitrotyrosine modification see [[Nitrotyrosine]]. | |||
The trinuclear center has two type 3 copper ions, that can be anti-ferromagnetically | == Relevance == | ||
Laccase from various fungi is used in adsorption of dyes from polluted environment</ref><ref>PMID:30997348</ref>. Laccases play an important role in food industry, paper and pulp industry, textile industry, synthetic chemistry, cosmetics, soil bioremediation and biodegradation of phenolic pollutants<ref>PMID:21755038</ref>. | |||
== Structural highlights == | |||
The trinuclear center of CotA laccase has two type 3 copper ions, that can be anti-ferromagnetically | |||
coupled through an hydroxyl moiety in between them, and one | coupled through an hydroxyl moiety in between them, and one | ||
type 2 copper ion.‡ The mononuclear copper is able to accept an | type 2 copper ion.‡ The mononuclear copper is able to accept an | ||
electron | electron from a variety of phenolic substrates and then transmit | ||
it to the trinuclear centre. | it to the trinuclear centre. | ||
==3D structures of | ==3D structures of laccase== | ||
[[Laccase 3D structures]] | |||
</StructureSection> | |||
== References == | |||
<references/> | |||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||