3n0s: Difference between revisions

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-{{Seed}}
-[[Image:3n0s.png|left|200px]]
-<!--
+==Crystal structure of BA2930 mutant (H183A) in complex with AcCoA==
-The line below this paragraph, containing "STRUCTURE_3n0s", creates the "Structure Box" on the page.
+<StructureSection load='3n0s' size='340' side='right'caption='[[3n0s]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3n0s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._Ames Bacillus anthracis str. Ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N0S FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-{{STRUCTURE_3n0s|  PDB=3n0s  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0s OCA], [https://pdbe.org/3n0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n0s RCSB], [https://www.ebi.ac.uk/pdbsum/3n0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0s ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A3P1UCA6_BACAN A0A3P1UCA6_BACAN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/3n0s_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n0s ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+For the last decade, worldwide efforts for the treatment of anthrax infection have focused on developing effective vaccines. Patients that are already infected are still treated traditionally using different types of standard antimicrobial agents. The most popular are antibiotics such as tetracyclines and fluoroquinolones. While aminoglycosides appear to be less effective antimicrobial agents than other antibiotics, synthetic aminoglycosides have been shown to act as potent inhibitors of anthrax lethal factor and may have potential application as antitoxins. Here, we present a structural analysis of the BA2930 protein, a putative aminoglycoside acetyltransferase, which may be a component of the bacterium's aminoglycoside resistance mechanism. The determined structures revealed details of a fold characteristic only for one other protein structure in the Protein Data Bank, namely, YokD from Bacillus subtilis. Both BA2930 and YokD are members of the Antibiotic_NAT superfamily (PF02522). Sequential and structural analyses showed that residues conserved throughout the Antibiotic_NAT superfamily are responsible for the binding of the cofactor acetyl coenzyme A. The interaction of BA2930 with cofactors was characterized by both crystallographic and binding studies.
-===Crystal structure of BA2930 mutant (H183A) in complex with AcCoA===
+Structural Analysis of a Putative Aminoglycoside N-Acetyltransferase from Bacillus anthracis.,Klimecka MM, Chruszcz M, Font J, Skarina T, Shumilin I, Onopryienko O, Porebski PJ, Cymborowski M, Zimmerman MD, Hasseman J, Glomski IJ, Lebioda L, Savchenko A, Edwards A, Minor W J Mol Biol. 2011 Jul 15;410(3):411-23. Epub 2011 May 13. PMID:21601576<ref>PMID:21601576</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-N0S is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0S OCA].
+<div class="pdbe-citations 3n0s" style="background-color:#fffaf0;"></div>
-[[Category: Bacillus anthracis]]
+== References ==
-[[Category: Anderson, W F.]]
+<references/>
-[[Category: Chruszcz, M.]]
+__TOC__
-[[Category: Cymborowski, M.]]
+</StructureSection>
-[[Category: Klimecka, M M.]]
+[[Category: Bacillus anthracis str. Ames]]
-[[Category: Minor, W.]]
+[[Category: Large Structures]]
-[[Category: Porebski, P J.]]
+[[Category: Anderson WF]]
-[[Category: 2 acyltransferase]]
+[[Category: Chruszcz M]]
-[[Category: Accoa]]
+[[Category: Cymborowski M]]
-[[Category: Center for structural genomics of infectious disease]]
+[[Category: Klimecka MM]]
-[[Category: Csgid]]
+[[Category: Minor W]]
-[[Category: Structural genomic]]
+[[Category: Porebski PJ]]
-[[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:37:36 2010''