3mmt: Difference between revisions

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-[[Image:3mmt.png|left|200px]]
-<!--
+==Crystal structure of fructose bisphosphate aldolase from Bartonella henselae, bound to fructose bisphosphate==
-The line below this paragraph, containing "STRUCTURE_3mmt", creates the "Structure Box" on the page.
+<StructureSection load='3mmt' size='340' side='right'caption='[[3mmt]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3mmt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MMT FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2FP:1,6-FRUCTOSE+DIPHOSPHATE+(LINEAR+FORM)'>2FP</scene></td></tr>
-{{STRUCTURE_3mmt|  PDB=3mmt  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mmt OCA], [https://pdbe.org/3mmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mmt RCSB], [https://www.ebi.ac.uk/pdbsum/3mmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mmt ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8L207_BARHN Q8L207_BARHN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/3mmt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mmt ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Fructose bisphosphate aldolase (FBPA) enzymes have been found in a broad range of eukaryotic and prokaryotic organisms. FBPA catalyses the cleavage of fructose 1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. The SSGCID has reported several FBPA structures from pathogenic sources, including the bacterium Brucella melitensis and the protozoan Babesia bovis. Bioinformatic analysis of the Bartonella henselae genome revealed an FBPA homolog. The B. henselae FBPA enzyme was recombinantly expressed and purified for X-ray crystallographic studies. The purified enzyme crystallized in the apo form but failed to diffract; however, well diffracting crystals could be obtained by cocrystallization in the presence of the native substrate fructose 1,6-bisphosphate. A data set to 2.35 A resolution was collected from a single crystal at 100 K. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 72.39, b = 127.71, c = 157.63 A. The structure was refined to a final free R factor of 22.2%. The structure shares the typical barrel tertiary structure and tetrameric quaternary structure reported for previous FBPA structures and exhibits the same Schiff base in the active site.
-===Crystal structure of fructose bisphosphate aldolase from Bartonella henselae, bound to fructose bisphosphate===
+Structure of fructose bisphosphate aldolase from Bartonella henselae bound to fructose 1,6-bisphosphate.,Gardberg A, Abendroth J, Bhandari J, Sankaran B, Staker B Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1051-4. Epub 2011 Aug 13. PMID:21904049<ref>PMID:21904049</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_21904049}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3mmt" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 21904049 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_21904049}}
-==About this Structure==
-[[3mmt]] is a 4 chain structure of [[Aldolase]] with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMT OCA].
 ==See Also==
-*[[Aldolase]]
+*[[Aldolase|Aldolase]]
+*[[Aldolase 3D structures|Aldolase 3D structures]]
-==Reference==
+== References ==
-<ref group="xtra">PMID:021904049</ref><references group="xtra"/>
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bartonella henselae]]
-[[Category: Fructose-bisphosphate aldolase]]
+[[Category: Large Structures]]
-[[Category: Ssgcid, Seattle Structural Genomics Center for Infectious Disease.]]
-[[Category: Aldolase]]
-[[Category: Hydrolase]]
-[[Category: Seattle structural genomics center for infectious disease]]
-[[Category: Ssgcid]]
-[[Category: Structural genomic]]