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| <SX load='5uz4' size='340' side='right' viewer='molstar' caption='[[5uz4]], [[Resolution|resolution]] 5.80Å' scene=''> | | <SX load='5uz4' size='340' side='right' viewer='molstar' caption='[[5uz4]], [[Resolution|resolution]] 5.80Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5uz4]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UZ4 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5uz4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UZ4 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GGM:3-O-(N-METHYLANTHRANILOYL)-BETA GAMMA-IMIDOGUANOSINE-5-TRIPHOSPHATE'>GGM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.8Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rsgA, engC, yjeQ, b4161, JW4122 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GGM:3-O-(N-METHYLANTHRANILOYL)-BETA GAMMA-IMIDOGUANOSINE-5-TRIPHOSPHATE'>GGM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uz4 OCA], [http://pdbe.org/5uz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uz4 RCSB], [http://www.ebi.ac.uk/pdbsum/5uz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uz4 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uz4 OCA], [https://pdbe.org/5uz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uz4 RCSB], [https://www.ebi.ac.uk/pdbsum/5uz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uz4 ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/RS12_ECO45 RS12_ECO45]] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.[HAMAP-Rule:MF_00403] [[http://www.uniprot.org/uniprot/RSGA_ECOLI RSGA_ECOLI]] One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late maturation steps of the functional core of the 30S ribosomal subunit (PubMed:18223068, PubMed:21102555, PubMed:21303937, PubMed:25904134, PubMed:27382067). Binds the 30S subunit contacting the head, platform, and rRNA helix 44, which may assist the last maturation stages (PubMed:21788480, PubMed:21960487). Removes RbfA from mature, but not immature 30S ribosomes in a GTP-dependent manner; 95% removal in the presence of GTP, 90% removal in GMP-PNP and 65% removal in the presence of GDP (PubMed:21102555, PubMed:25904134). Circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (PubMed:12220175). Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96-fold increase in kcat) ribosomes (PubMed:14973029). Mature 30S ribosomes stimulate intrinsic GTPase more than do immature 30S ribosomes (PubMed:25904134). Ribosome-associated GTPase activity is stimulated by RbfA (PubMed:21102555). The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin (PubMed:15466596) streptomycin and spectinomycin (PubMed:15828870). This inhibition is not due to competition for binding sites on the 30S or 70S ribosome (PubMed:15828870).<ref>PMID:12220175</ref> <ref>PMID:14973029</ref> <ref>PMID:15466596</ref> <ref>PMID:15828870</ref> <ref>PMID:21102555</ref> <ref>PMID:25904134</ref> <ref>PMID:18223068</ref> <ref>PMID:27382067</ref> [[http://www.uniprot.org/uniprot/RS8_ECO45 RS8_ECO45]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit. [[http://www.uniprot.org/uniprot/RS18_ECO45 RS18_ECO45]] Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit. [[http://www.uniprot.org/uniprot/RS3_ECO45 RS3_ECO45]] Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation. [[http://www.uniprot.org/uniprot/RS19_ECO45 RS19_ECO45]] Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. [[http://www.uniprot.org/uniprot/RS17_ECO45 RS17_ECO45]] One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. [[http://www.uniprot.org/uniprot/RS14_ECO45 RS14_ECO45]] Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. [[http://www.uniprot.org/uniprot/RS7_ECOLI RS7_ECOLI]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.<ref>PMID:2461734</ref> Protein S7 is also a translational repressor protein; it regulates the expression of the str operon members to different degrees by binding to its mRNA.<ref>PMID:2461734</ref> [[http://www.uniprot.org/uniprot/RS4_ECO45 RS4_ECO45]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. With S5 and S12 plays an important role in translational accuracy. [[http://www.uniprot.org/uniprot/RS10_ECO45 RS10_ECO45]] Involved in the binding of tRNA to the ribosomes. [[http://www.uniprot.org/uniprot/RS13_ECO57 RS13_ECO57]] Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the E.coli 70S ribosome in the initiation state it has been modeled to contact the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; bridge B1a is broken in the model with bound EF-G, while the protein-protein contacts between S13 and L5 in B1b change. Contacts the tRNAs in the A and P sites (By similarity). [[http://www.uniprot.org/uniprot/RS15_ECO57 RS15_ECO57]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA (By similarity). Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome (By similarity). [[http://www.uniprot.org/uniprot/RS5_ECO57 RS5_ECO57]] With S4 and S12 plays an important role in translational accuracy. Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body. [[http://www.uniprot.org/uniprot/RS20_ECO45 RS20_ECO45]] Binds directly to 16S ribosomal RNA. [[http://www.uniprot.org/uniprot/RS11_ECO45 RS11_ECO45]] Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome. [[http://www.uniprot.org/uniprot/RS6_ECOLI RS6_ECOLI]] Binds together with S18 to 16S ribosomal RNA.[HAMAP-Rule:MF_00360] | | [https://www.uniprot.org/uniprot/RS3_ECOLI RS3_ECOLI] Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity).<ref>PMID:15652481</ref> Plays a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp.<ref>PMID:15652481</ref> |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Bacillus coli migula 1895]]
| |
| [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Guarne, A]] | | [[Category: Guarne A]] |
| [[Category: Ortega, J]] | | [[Category: Ortega J]] |
| [[Category: Razi, A]] | | [[Category: Razi A]] |
| [[Category: 30s subunit]]
| |
| [[Category: Ribosome assembly]]
| |
| [[Category: Ribosome-hydrolase complex]]
| |
| [[Category: Rsga protein]]
| |
| [[Category: Yjeq protein]]
| |