3hot: Difference between revisions

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-{{Seed}}
-[[Image:3hot.jpg|left|200px]]
-<!--
+==Crystal structure of the Mos1 mariner paired end complex with Mn==
-The line below this paragraph, containing "STRUCTURE_3hot", creates the "Structure Box" on the page.
+<StructureSection load='3hot' size='340' side='right'caption='[[3hot]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3hot]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_mauritiana Drosophila mauritiana] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HOT FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_3hot|  PDB=3hot  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hot OCA], [https://pdbe.org/3hot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hot RCSB], [https://www.ebi.ac.uk/pdbsum/3hot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hot ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MOS1T_DROMA MOS1T_DROMA] Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ho/3hot_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hot ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A key step in cut-and-paste DNA transposition is the pairing of transposon ends before the element is excised and inserted at a new site in its host genome. Crystallographic analyses of the paired-end complex (PEC) formed from precleaved transposon ends and the transposase of the eukaryotic element Mos1 reveals two parallel ends bound to a dimeric enzyme. The complex has a trans arrangement, with each transposon end recognized by the DNA binding region of one transposase monomer and by the active site of the other monomer. Two additional DNA duplexes in the crystal indicate likely binding sites for flanking DNA. Biochemical data provide support for a model of the target capture complex and identify Arg186 to be critical for target binding. Mixing experiments indicate that a transposase dimer initiates first-strand cleavage and suggest a pathway for PEC formation.
-===Crystal structure of the Mos1 mariner paired end complex with Mn===
+Molecular architecture of the Mos1 paired-end complex: the structural basis of DNA transposition in a eukaryote.,Richardson JM, Colloms SD, Finnegan DJ, Walkinshaw MD Cell. 2009 Sep 18;138(6):1096-108. PMID:19766564<ref>PMID:19766564</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3hot" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-HOT is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_mauritiana Drosophila mauritiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HOT OCA].
+*[[Transposase 3D structures|Transposase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila mauritiana]]
-[[Category: Richardson, J M.]]
+[[Category: Large Structures]]
-[[Category: Walkinshaw, M D.]]
+[[Category: Synthetic construct]]
-[[Category: Dna binding protein/dna complex]]
+[[Category: Richardson JM]]
-[[Category: Inverted repeat dna]]
+[[Category: Walkinshaw MD]]
-[[Category: Protein-dna complex]]
-[[Category: Synaptic complex]]
-[[Category: Transferase]]
-[[Category: Transposase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 23 08:58:18 2009''