3h6c: Difference between revisions

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-[[Image:3h6c.png|left|200px]]
-<!--
+==Crystal structure of human alpha-defensin 1 (Mutant Gln22Ala)==
-The line below this paragraph, containing "STRUCTURE_3h6c", creates the "Structure Box" on the page.
+<StructureSection load='3h6c' size='340' side='right'caption='[[3h6c]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3h6c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H6C FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-{{STRUCTURE_3h6c|  PDB=3h6c  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h6c OCA], [https://pdbe.org/3h6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h6c RCSB], [https://www.ebi.ac.uk/pdbsum/3h6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h6c ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DEF1_HUMAN DEF1_HUMAN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/3h6c_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h6c ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We performed a comprehensive alanine scan of human alpha-defensin HNP1 and tested the ability of the resulting analogs to kill Staphylococcus aureus, inhibit anthrax lethal factor, and bind human immunodeficiency virus-1 gp120. By far, the most deleterious mutation for all of these functions was W26A. The activities lost by W26A-HNP1 were restored progressively by replacing W26 with non-coded, straight-chain aliphatic amino acids of increasing chain length. The hydrophobicity of residue 26 also correlated with the ability of the analogs to bind immobilized wild type HNP1 and to undergo further self-association. Thus, the hydrophobicity of residue 26 is not only a key determinant of the direct interactions of HNP1 with target molecules, but it also governs the ability of this peptide to form dimers and more complex quaternary structures at micromolar concentrations. Although all defensin peptides are cationic, their amphipathicity is at least as important as their positive charge in enabling them to participate in innate host defense.
-===Crystal structure of human alpha-defensin 1 (Mutant Gln22Ala)===
+Trp-26 imparts functional versatility to human alpha-defensin HNP1.,Wei G, Pazgier M, de Leeuw E, Rajabi M, Li J, Zou G, Jung G, Yuan W, Lu WY, Lehrer RI, Lu W J Biol Chem. 2010 May 21;285(21):16275-85. Epub 2010 Mar 10. PMID:20220136<ref>PMID:20220136</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3h6c" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20220136}}, adds the Publication Abstract to the page
+*[[Defensin 3D structures|Defensin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20220136 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20220136}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Homo sapiens]]
-[[3h6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H6C OCA].
+[[Category: Large Structures]]
+[[Category: Lu W]]
-==Reference==
+[[Category: Pazgier M]]
-<ref group="xtra">PMID:020220136</ref><references group="xtra"/>
-[[Category: Lu, W.]]
-[[Category: Pazgier, M.]]
-[[Category: Antibiotic]]
-[[Category: Antimicrobial]]
-[[Category: Antimicrobial peptide]]
-[[Category: Antimicrobial protein]]
-[[Category: Antiviral defense]]
-[[Category: Defensin]]
-[[Category: Disulfide bond]]
-[[Category: Fungicide]]
-[[Category: Phosphoprotein]]
-[[Category: Q22a mutant of human alpha defensin 1 q22a mutant of human neutrophil peptide 1]]
-[[Category: Secreted]]