3h0g: Difference between revisions

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-{{Seed}}
-[[Image:3h0g.png|left|200px]]
-<!--
+==RNA Polymerase II from Schizosaccharomyces pombe==
-The line below this paragraph, containing "STRUCTURE_3h0g", creates the "Structure Box" on the page.
+<StructureSection load='3h0g' size='340' side='right'caption='[[3h0g]], [[Resolution|resolution]] 3.65&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3h0g]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H0G FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.65&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_3h0g|  PDB=3h0g  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h0g OCA], [https://pdbe.org/3h0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h0g RCSB], [https://www.ebi.ac.uk/pdbsum/3h0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h0g ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPB9_SCHPO RPB9_SCHPO]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h0/3h0g_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h0g ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The second structure of a eukaryotic RNA polymerase II so far determined, that of the enzyme from the fission yeast Schizosaccharomyces pombe, is reported here. Comparison with the previous structure of the enzyme from the budding yeast Saccharomyces cerevisiae reveals differences in regions implicated in start site selection and transcription factor interaction. These aspects of the transcription mechanism differ between S. pombe and S. cerevisiae, but are conserved between S. pombe and humans. Amino acid changes apparently responsible for the structural differences are also conserved between S. pombe and humans, suggesting that the S. pombe structure may be a good surrogate for that of the human enzyme.
-===RNA Polymerase II from Schizosaccharomyces pombe===
+Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.,Spahr H, Calero G, Bushnell DA, Kornberg RD Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9185-90. Epub 2009 May 20. PMID:19458260<ref>PMID:19458260</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3h0g" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-H0G is a 24 chains structure of sequences from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H0G OCA].
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:19458260</ref><references group="xtra"/>
+__TOC__
-[[Category: DNA-directed RNA polymerase]]
+</StructureSection>
-[[Category: Schizosaccharomyces pombe]]
+[[Category: Large Structures]]
-[[Category: Bushnell, D A.]]
+[[Category: Schizosaccharomyces pombe 972h-]]
-[[Category: Calero, G.]]
+[[Category: Bushnell DA]]
-[[Category: Kornberg, R D.]]
+[[Category: Calero G]]
-[[Category: Spahr, H.]]
+[[Category: Kornberg RD]]
-[[Category: Dna-binding]]
+[[Category: Spahr H]]
-[[Category: Dna-directed rna polymerase]]
-[[Category: Magnesium]]
-[[Category: Metal-binding]]
-[[Category: Multi-protein complex]]
-[[Category: Nucleotidyltransferase]]
-[[Category: Nucleus]]
-[[Category: Phosphoprotein]]
-[[Category: Polymerase]]
-[[Category: Transcription]]
-[[Category: Transferase]]
-[[Category: Zinc]]
-[[Category: Zinc-finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Sep 21 16:57:48 2009''