5mcs: Difference between revisions
New page: '''Unreleased structure''' The entry 5mcs is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures |
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==Solution structure and dynamics of the outer membrane cytochrome OmcF from Geobacter sulfurreducens== | |||
<StructureSection load='5mcs' size='340' side='right'caption='[[5mcs]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5mcs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens_PCA Geobacter sulfurreducens PCA]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MCS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mcs OCA], [https://pdbe.org/5mcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mcs RCSB], [https://www.ebi.ac.uk/pdbsum/5mcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mcs ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q74AE4_GEOSL Q74AE4_GEOSL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Gene knock-out studies on Geobacter sulfurreducens cells showed that the outer membrane-associated monoheme cytochrome OmcF is involved in respiratory pathways leading to the extracellular reduction of Fe(III) and U(VI). In addition, microarray analysis of an OmcF-deficient mutant revealed that many of the genes with decreased transcript level were those whose expression is up-regulated in cells grown with a graphite electrode as electron acceptor, suggesting that OmcF also regulates the electron transfer to electrode surfaces and the concomitant electricity production by G. sulfurreducens in microbial fuel cells. 15N,13C-labeled OmcF was produced and NMR spectroscopy was used to determine the solution structure of the protein in the fully reduced state and the pH-dependent conformational changes. In addition, 15N relaxation NMR experiments were used to characterize the overall and internal backbone dynamics of OmcF. The structure obtained is well defined, with an average pairwise root mean square deviation of 0.37 A for the backbone atoms and 0.98 A for all heavy atoms. For the first time a solution structure and the protein motions were determined for an outer membrane cytochrome from G. sulfurreducens, which constitutes an important step to understand the extracellular electron transfer mechanism in Geobacter cells. | |||
Solution structure and dynamics of the outer membrane cytochrome OmcF from Geobacter sulfurreducens.,Dantas JM, Silva MA, Pantoja-Uceda D, Turner DL, Bruix M, Salgueiro CA Biochim Biophys Acta. 2017 Apr 1. pii: S0005-2728(17)30056-7. doi:, 10.1016/j.bbabio.2017.03.007. PMID:28377068<ref>PMID:28377068</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5mcs" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Geobacter sulfurreducens PCA]] | |||
[[Category: Large Structures]] | |||
[[Category: Bruix M]] | |||
[[Category: Dantas JM]] | |||
[[Category: Morgado L]] | |||
[[Category: Pantoja-Uceda D]] | |||
[[Category: Salgueiro CA]] | |||
[[Category: Silva MA]] | |||
[[Category: Turner DL]] | |||
Latest revision as of 12:10, 23 October 2024
Solution structure and dynamics of the outer membrane cytochrome OmcF from Geobacter sulfurreducensSolution structure and dynamics of the outer membrane cytochrome OmcF from Geobacter sulfurreducens
Structural highlights
FunctionPublication Abstract from PubMedGene knock-out studies on Geobacter sulfurreducens cells showed that the outer membrane-associated monoheme cytochrome OmcF is involved in respiratory pathways leading to the extracellular reduction of Fe(III) and U(VI). In addition, microarray analysis of an OmcF-deficient mutant revealed that many of the genes with decreased transcript level were those whose expression is up-regulated in cells grown with a graphite electrode as electron acceptor, suggesting that OmcF also regulates the electron transfer to electrode surfaces and the concomitant electricity production by G. sulfurreducens in microbial fuel cells. 15N,13C-labeled OmcF was produced and NMR spectroscopy was used to determine the solution structure of the protein in the fully reduced state and the pH-dependent conformational changes. In addition, 15N relaxation NMR experiments were used to characterize the overall and internal backbone dynamics of OmcF. The structure obtained is well defined, with an average pairwise root mean square deviation of 0.37 A for the backbone atoms and 0.98 A for all heavy atoms. For the first time a solution structure and the protein motions were determined for an outer membrane cytochrome from G. sulfurreducens, which constitutes an important step to understand the extracellular electron transfer mechanism in Geobacter cells. Solution structure and dynamics of the outer membrane cytochrome OmcF from Geobacter sulfurreducens.,Dantas JM, Silva MA, Pantoja-Uceda D, Turner DL, Bruix M, Salgueiro CA Biochim Biophys Acta. 2017 Apr 1. pii: S0005-2728(17)30056-7. doi:, 10.1016/j.bbabio.2017.03.007. PMID:28377068[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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