3fpx: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:3fpx.jpg|left|200px]]


<!--
==Native fungus laccase from Trametes hirsuta==
The line below this paragraph, containing "STRUCTURE_3fpx", creates the "Structure Box" on the page.
<StructureSection load='3fpx' size='340' side='right'caption='[[3fpx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3fpx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trametes_hirsuta Trametes hirsuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
{{STRUCTURE_3fpx|  PDB=3fpx  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpx OCA], [https://pdbe.org/3fpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpx RCSB], [https://www.ebi.ac.uk/pdbsum/3fpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B2L9C1_TRAHI B2L9C1_TRAHI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpx ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Laccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map.


===Native fungus laccase from Trametes hirsuta===
Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus.,Pegasova TV, Zwart P, Koroleva OV, Stepanova EV, Rebrikov DV, Lamzin VS Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. Epub 2003, Jul 23. PMID:012876350<ref>PMID:012876350</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3fpx" style="background-color:#fffaf0;"></div>


<!--
==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_12876350}}, adds the Publication Abstract to the page
*[[Laccase 3D structures|Laccase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 12876350 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_12876350}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3FPX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Trametes_hirsuta Trametes hirsuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPX OCA].
 
==Reference==
Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus., Pegasova TV, Zwart P, Koroleva OV, Stepanova EV, Rebrikov DV, Lamzin VS, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876350 12876350]
[[Category: Laccase]]
[[Category: Single protein]]
[[Category: Trametes hirsuta]]
[[Category: Trametes hirsuta]]
[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
[[Category: Cherkashin EA]]
[[Category: 4-copper protein]]
[[Category: Fedorova TV]]
[[Category: Beta sheet]]
[[Category: Koroleva OV]]
[[Category: Copper]]
[[Category: Kurzeev SA]]
[[Category: Metal-binding]]
[[Category: Lamzin VS]]
[[Category: Oxidoreductase]]
[[Category: Polyakov KM]]
 
[[Category: Stepanova EV]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb  4 20:23:59 2009''
[[Category: Strokopytov BV]]

Latest revision as of 12:09, 30 October 2024

Native fungus laccase from Trametes hirsutaNative fungus laccase from Trametes hirsuta

Structural highlights

3fpx is a 1 chain structure with sequence from Trametes hirsuta. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B2L9C1_TRAHI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Laccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map.

Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus.,Pegasova TV, Zwart P, Koroleva OV, Stepanova EV, Rebrikov DV, Lamzin VS Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. Epub 2003, Jul 23. PMID:012876350[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Pegasova TV, Zwart P, Koroleva OV, Stepanova EV, Rebrikov DV, Lamzin VS. Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus. Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. Epub 2003, Jul 23. PMID:12876350

3fpx, resolution 1.80Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3fpx&oldid=4207006"