Isoelectric point: Difference between revisions
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***[http://molbiol-tools.ca/Translation.htm Another online site] lists several tools that may be useful depending on the state of the nucleotide sequence you have available. | ***[http://molbiol-tools.ca/Translation.htm Another online site] lists several tools that may be useful depending on the state of the nucleotide sequence you have available. | ||
== | ==Protein Calculator== | ||
#First, get the one-letter amino acid sequence of your protein of interest. | #First, get the one-letter amino acid sequence of your protein of interest. | ||
##See above for some methods. | ##See above for some methods. | ||
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#Check the first box under Charge at the right, and click Submit Query. | #Check the first box under Charge at the right, and click Submit Query. | ||
== | ==Prot-pi== | ||
"Prot pi | Protein Tool calculates isoelectric point and net charge of proteins, as well as the exact mass and the absorption coefficient using the amino acid sequence." | |||
*Go to [https://www.protpi.ch/Calculator/ProteinTool Prot-pi]] and follow instructions. | |||
==EMBL WWW Gateway to Isoelectric Point Service== | |||
<font color='red'>The EMBL Gateway to pI appears to be out of service in May, 2013. Use the alternative below.</font> | <font color='red'>The EMBL Gateway to pI appears to be out of service in May, 2013. Use the alternative below.</font> | ||