3cw2: Difference between revisions
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<StructureSection load='3cw2' size='340' side='right'caption='[[3cw2]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='3cw2' size='340' side='right'caption='[[3cw2]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3cw2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3cw2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CW2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cw2 OCA], [https://pdbe.org/3cw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cw2 RCSB], [https://www.ebi.ac.uk/pdbsum/3cw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cw2 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cw2 OCA], [https://pdbe.org/3cw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cw2 RCSB], [https://www.ebi.ac.uk/pdbsum/3cw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cw2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/IF2G_SACS2 IF2G_SACS2] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.[HAMAP-Rule:MF_00119] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/3cw2_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/3cw2_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Blaesi | [[Category: Saccharolobus solfataricus]] | ||
[[Category: Fedorov | [[Category: Blaesi U]] | ||
[[Category: Garber | [[Category: Fedorov RV]] | ||
[[Category: Manstein | [[Category: Garber MB]] | ||
[[Category: Nikonov | [[Category: Manstein DJ]] | ||
[[Category: Nikonov | [[Category: Nikonov OS]] | ||
[[Category: Nikulin | [[Category: Nikonov SV]] | ||
[[Category: Stolboushkina | [[Category: Nikulin AD]] | ||
[[Category: Stolboushkina EA]] | |||
Latest revision as of 08:44, 17 October 2024
Crystal structure of the intact archaeal translation initiation factor 2 from Sulfolobus solfataricus .Crystal structure of the intact archaeal translation initiation factor 2 from Sulfolobus solfataricus .
Structural highlights
FunctionIF2G_SACS2 eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.[HAMAP-Rule:MF_00119] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedIn Eukarya and Archaea, translation initiation factor 2 (eIF2/aIF2), which contains three subunits (alpha, beta, and gamma), is pivotal for binding of charged initiator tRNA to the small ribosomal subunit. The crystal structure of the full-sized heterotrimeric aIF2 from Sulfolobus solfataricus in the nucleotide-free form has been determined at 2.8-A resolution. Superposition of four molecules in the asymmetric unit of the crystal and the comparison of the obtained structures with the known structures of the aIF2alphagamma and aIF2betagamma heterodimers revealed high conformational flexibility in the alpha- and beta-subunits. In fact, the full-sized aIF2 consists of a rigid central part, formed by the gamma-subunit, domain 3 of the alpha-subunit, and the N-terminal alpha-helix of the beta-subunit, and two mobile "wings," formed by domains 1 and 2 of the alpha-subunit, the central part, and the zinc-binding domain of the beta-subunit. High structural flexibility of the wings is probably required for interaction of aIF2 with the small ribosomal subunit. Comparative analysis of all known structures of the gamma-subunit alone and within the heterodimers and heterotrimers in nucleotide-bound and nucleotide-free states shows that the conformations of switch 1 and switch 2 do not correlate with the assembly or nucleotide states of the protein. Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits.,Stolboushkina E, Nikonov S, Nikulin A, Blasi U, Manstein DJ, Fedorov R, Garber M, Nikonov O J Mol Biol. 2008 Oct 10;382(3):680-91. Epub 2008 Jul 22. PMID:18675278[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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