3bfh: Difference between revisions

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-{{Seed}}
-[[Image:3bfh.png|left|200px]]
-<!--
+==Crystal structure of a pheromone binding protein from Apis mellifera in complex with hexadecanoic acid==
-The line below this paragraph, containing "STRUCTURE_3bfh", creates the "Structure Box" on the page.
+<StructureSection load='3bfh' size='340' side='right'caption='[[3bfh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3bfh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BFH FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-{{STRUCTURE_3bfh|  PDB=3bfh  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bfh OCA], [https://pdbe.org/3bfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bfh RCSB], [https://www.ebi.ac.uk/pdbsum/3bfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bfh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9U9J6_APIME Q9U9J6_APIME]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/3bfh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bfh ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The behavior of insects and their perception of their surroundings are driven, in a large part, by odorants and pheromones. This is especially true for social insects, such as the honey bee, where the queen controls the development and the caste status of the other individuals. Pheromone perception is a complex phenomenon relying on a cascade of recognition events, initiated in antennae by pheromone recognition by a pheromone-binding protein and finishing with signal transduction at the axon membrane level. With to the objective of deciphering this initial step, we have determined the structures of the bee antennal pheromone-binding protein (ASP1) in the apo form and in complex with the main component of the queen mandibular pheromonal mixture, 9-keto-2(E)-decenoic acid (9-ODA) and with nonpheromonal components. In the apo protein, the C terminus obstructs the binding site. In contrast, ASP1 complexes have different open conformations, depending on the ligand shape, leading to different volumes of the binding cavity. The binding site integrity depends on the C terminus (111-119) conformation, which involves the interplay of two factors; i.e. the presence of a ligand and a low pH. Ligand binding to ASP1 is favored by low pH, opposite to what is observed with other pheromone-binding proteins, such as those of Bombyx mori and Anopheles gambiae.
-===Crystal structure of a pheromone binding protein from Apis mellifera in complex with hexadecanoic acid===
+Structural basis of the honey bee PBP pheromone and pH-induced conformational change.,Pesenti ME, Spinelli S, Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C J Mol Biol. 2008 Jun 27;380(1):158-69. Epub 2008 Apr 27. PMID:18508083<ref>PMID:18508083</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3bfh" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18508083}}, adds the Publication Abstract to the page
+*[[Pheromone binding protein|Pheromone binding protein]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18508083 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18508083}}
+__TOC__
+</StructureSection>
-==About this Structure==
-BFH is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFH OCA].
-==Reference==
-<ref group="xtra">PMID:18508083</ref><references group="xtra"/>
-[[Category: Apis mellifera]]
-[[Category: Bezirard, V.]]
-[[Category: Briand, L.]]
-[[Category: Cambillau, C.]]
-[[Category: Pernollet, J C.]]
-[[Category: Pesenti, M E.]]
-[[Category: Spinelli, S.]]
-[[Category: Tegoni, M.]]
 [[Category: Apis mellifera]]
-[[Category: Hexadecanoic acid]]
+[[Category: Large Structures]]
-[[Category: Honeybee]]
+[[Category: Bezirard V]]
-[[Category: Pheromone binding protein]]
+[[Category: Briand L]]
-[[Category: Pheromone-binding protein]]
+[[Category: Cambillau C]]
-[[Category: Queen mandibular pheromone]]
+[[Category: Pernollet JC]]
-[[Category: Signal transduction]]
+[[Category: Pesenti ME]]
+[[Category: Spinelli S]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:07:27 2009''
+[[Category: Tegoni M]]