2vu8: Difference between revisions
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<StructureSection load='2vu8' size='340' side='right' caption='[[2vu8]], [[Resolution|resolution]] 1.80Å' scene=''> | ==Crystal structure of an insect inhibitor with a fungal trypsin== | ||
<StructureSection load='2vu8' size='340' side='right'caption='[[2vu8]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vu8]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2vu8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum] and [https://en.wikipedia.org/wiki/Locusta_migratoria Locusta migratoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VU8 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vu8 OCA], [https://pdbe.org/2vu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vu8 RCSB], [https://www.ebi.ac.uk/pdbsum/2vu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vu8 ProSAT]</span></td></tr> | ||
</table> | |||
<table> | == Function == | ||
[https://www.uniprot.org/uniprot/TRYP_FUSOX TRYP_FUSOX] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vu8_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vu8_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vu8 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2vu8" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Trypsin|Trypsin]] | *[[Trypsin 3D structures|Trypsin 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 33: | Line 36: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Fusarium oxysporum]] | [[Category: Fusarium oxysporum]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Locusta migratoria]] | ||
[[Category: | [[Category: Kellenberger C]] | ||
[[Category: | [[Category: Leone P]] | ||
[[Category: | [[Category: Roussel A]] | ||
Latest revision as of 12:33, 6 November 2024
Crystal structure of an insect inhibitor with a fungal trypsinCrystal structure of an insect inhibitor with a fungal trypsin
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedPrevious studies have shown that the trypsin inhibitors LMPI-1, LMPI-3 and SGTI from locusts display an unusual species selectivity. They inhibit locust, crayfish and fungal trypsins several orders of magnitude more efficiently than bovine trypsin. In contrast, the chymotrypsin inhibitors from the same family, LMPI-2 and SGCI, are active towards mammalian enzymes. The crystal structures of a variant of LMPI-1 and of LMPI-2 in complex with bovine chymotrypsin have revealed subtle structural differences between the trypsin and chymotrypsin inhibitors. In a previous report, it was proposed that Pro173 of bovine trypsin is responsible for the weak inhibitory activity of LMPI-1 and LMPI-3. A fungal trypsin from Fusarium oxysporum contains Gly173 instead of Pro173 and has been shown to be strongly inhibited by LMPI-1 and LMPI-3. To explore the structural features that are responsible for this property, the crystal structure of the complex between LMPI-3 and F. oxysporum trypsin was determined at 1.8 A resolution. This study indicates that this small inhibitor interacts with the protease through the reactive site P3-P4' and the P10-P6 residues. Comparison of this complex with the SGTI-crayfish trypsin and BPTI-bovine trypsin complexes reinforces this hypothesis on the role of residue 173 of trypsin in species selectivity. Structure of Locusta migratoria protease inhibitor 3 (LMPI-3) in complex with Fusarium oxysporum trypsin.,Leone P, Roussel A, Kellenberger C Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1165-71. Epub 2008, Oct 18. PMID:19020355[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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