2reg: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:2reg.png|left|200px]]
-<!--
+==ABC-transporter choline binding protein in complex with choline==
-The line below this paragraph, containing "STRUCTURE_2reg", creates the "Structure Box" on the page.
+<StructureSection load='2reg' size='340' side='right'caption='[[2reg]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2reg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2REG FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene></td></tr>
-{{STRUCTURE_2reg|  PDB=2reg  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2reg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reg OCA], [https://pdbe.org/2reg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2reg RCSB], [https://www.ebi.ac.uk/pdbsum/2reg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2reg ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q92N37_RHIME Q92N37_RHIME]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/2reg_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2reg ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The ATP-binding cassette transporter ChoVWX is one of several choline import systems operating in Sinorhizobium meliloti. Here fluorescence-based ligand binding assays were used to quantitate substrate binding by the periplasmic ligand-binding protein ChoX. These data confirmed that ChoX recognizes choline and acetylcholine with high and medium affinity, respectively. We also report the crystal structures of ChoX in complex with either choline or acetylcholine. These structural investigations revealed an architecture of the ChoX binding pocket and mode of substrate binding similar to that reported previously for several compatible solute-binding proteins. Additionally the ChoX-acetylcholine complex permitted a detailed structural comparison with the carbamylcholine-binding site of the acetylcholine-binding protein from the mollusc Lymnaea stagnalis. In addition to the two liganded structures of ChoX, we were also able to solve the crystal structure of ChoX in a closed, substrate-free conformation that revealed an architecture of the ligand-binding site that is superimposable to the closed, ligand-bound form of ChoX. This structure is only the second of its kind and raises the important question of how ATP-binding cassette transporters are capable of distinguishing liganded and unliganded-closed states of the binding protein.
-===ABC-transporter choline binding protein in complex with choline===
+Crystal structures of the choline/acetylcholine substrate-binding protein ChoX from Sinorhizobium meliloti in the liganded and unliganded-closed states.,Oswald C, Smits SH, Hoing M, Sohn-Bosser L, Dupont L, Le Rudulier D, Schmitt L, Bremer E J Biol Chem. 2008 Nov 21;283(47):32848-59. Epub 2008 Sep 8. PMID:18779321<ref>PMID:18779321</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2reg" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18779321}}, adds the Publication Abstract to the page
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18779321 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18779321}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-REG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REG OCA].
-==Reference==
-Crystal structures of the choline/acetylcholine substrate binding protein ChoX from Sinorhizobium meliloti in the liganded and unliganded closed states., Oswald C, Smits SH, Hoing M, Sohn-Bosser L, Dupont L, Le Rudulier D, Schmitt L, Bremer E, J Biol Chem. 2008 Sep 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18779321 18779321]
-[[Category: Single protein]]
 [[Category: Sinorhizobium meliloti]]
-[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
+[[Category: Bremer E]]
-[[Category: Abc-transporter]]
+[[Category: Hoeing M]]
-[[Category: Aromatic box]]
+[[Category: Oswald C]]
-[[Category: Transport protein]]
+[[Category: Schimtt L]]
-[[Category: Type ii binding protein]]
+[[Category: Smits SHJ]]
+[[Category: Sohn-Boeser L]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 14 20:09:43 2009''