2hrs: Difference between revisions

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[[Image:2hrs.png|left|200px]]


{{STRUCTURE_2hrs|  PDB=2hrs  |  SCENE=  }}
==Crystal structure of L42H V224H design intermediate for GFP metal ion reporter==
 
<StructureSection load='2hrs' size='340' side='right'caption='[[2hrs]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
===Crystal structure of L42H V224H design intermediate for GFP metal ion reporter===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[2hrs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HRS FirstGlance]. <br>
 
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
==About this Structure==
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
[[2hrs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HRS OCA].  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hrs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hrs OCA], [https://pdbe.org/2hrs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hrs RCSB], [https://www.ebi.ac.uk/pdbsum/2hrs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hrs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/2hrs_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hrs ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
__TOC__
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
</StructureSection>
*[[Sandbox104|Sandbox104]]
*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: Barondeau, D P.]]
[[Category: Large Structures]]
[[Category: Getzoff, E D.]]
[[Category: Barondeau DP]]
[[Category: Tainer, J A.]]
[[Category: Getzoff ED]]
[[Category: Tubbs, J L.]]
[[Category: Tainer JA]]
[[Category: Fluorophore]]
[[Category: Tubbs JL]]
[[Category: Gfp]]
[[Category: Intermediate]]
[[Category: Luminescent protein]]
[[Category: Metal site design]]
[[Category: Reporter]]

Latest revision as of 04:02, 21 November 2024

Crystal structure of L42H V224H design intermediate for GFP metal ion reporterCrystal structure of L42H V224H design intermediate for GFP metal ion reporter

Structural highlights

2hrs is a 1 chain structure with sequence from Aequorea victoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2hrs, resolution 1.40Å

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