2ra4: Difference between revisions

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[[Image:2ra4.jpg|left|200px]]


{{Structure
==Crystal Structure of Human Monocyte Chemoattractant Protein 4 (MCP-4/CCL13)==
|PDB= 2ra4 |SIZE=350|CAPTION= <scene name='initialview01'>2ra4</scene>, resolution 1.70&Aring;
<StructureSection load='2ra4' size='340' side='right'caption='[[2ra4]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+76'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+77'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+B+76'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+B+77'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+78'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+B+78'>AC6</scene> and <scene name='pdbsite=AC7:Tfa+Binding+Site+For+Residue+B+79'>AC7</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETYL+GROUP'>TFA</scene>
<table><tr><td colspan='2'>[[2ra4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RA4 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
|GENE= CCL13, MCP4, NCC1, SCYA13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ra4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ra4 OCA], [https://pdbe.org/2ra4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ra4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ra4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ra4 ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ra4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ra4 OCA], [http://www.ebi.ac.uk/pdbsum/2ra4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ra4 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/CCL13_HUMAN CCL13_HUMAN] Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2ra4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ra4 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Monocyte chemoattractant proteins (MCPs) belong to the CC chemokine family and are involved in many (patho)physiological processes characterized by mononuclear cell infiltration, including tissue remodeling, atherosclerosis and cancer metastasis. Here, the crystal structure of human monocyte chemoattractant protein 4 (MCP-4) refined at 1.70 A resolution is reported with crystallographic values R = 0.180 and R free = 0.212. The overall MCP-4 fold reveals the typical tertiary features of the CC chemokine family. A central three-stranded antiparallel beta-sheet is C-terminally flanked by an overlaying alpha-helix, while the N-terminal part of the molecule forms an extended loop that is anchored to the rest of the molecule via two disulfide bridges, Cys11-Cys35 and Cys12-Cys51. The crystal packing suggests the existence of MCP-4 dimers with a dimerization interface similar to those previously reported for the X-ray structures of MCP-1 and MCP-2.


'''Crystal Structure of Human Monocyte Chemoattractant Protein 4 (MCP-4/CCL13)'''
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13).,Barinka C, Prahl A, Lubkowski J Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):273-8. Epub 2008, Feb 20. PMID:18323622<ref>PMID:18323622</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ra4" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
Monocyte chemoattractant proteins (MCPs) belong to the CC chemokine family and are involved in many (patho)physiological processes characterized by mononuclear cell infiltration, including tissue remodeling, atherosclerosis and cancer metastasis. Here, the crystal structure of human monocyte chemoattractant protein 4 (MCP-4) refined at 1.70 A resolution is reported with crystallographic values R = 0.180 and R(free) = 0.212. The overall MCP-4 fold reveals the typical tertiary features of the CC chemokine family. A central three-stranded antiparallel beta-sheet is C-terminally flanked by an overlaying alpha-helix, while the N-terminal part of the molecule forms an extended loop that is anchored to the rest of the molecule via two disulfide bridges, Cys11-Cys35 and Cys12-Cys51. The crystal packing suggests the existence of MCP-4 dimers with a dimerization interface similar to those previously reported for the X-ray structures of MCP-1 and MCP-2.
*[[Monocyte chemoattractant protein|Monocyte chemoattractant protein]]
 
*[[Multiple sclerosis|Multiple sclerosis]]
==About this Structure==
== References ==
2RA4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA4 OCA].
<references/>
 
__TOC__
==Reference==
</StructureSection>
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13)., Barinka C, Prahl A, Lubkowski J, Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):273-8. Epub 2008, Feb 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18323622 18323622]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Barinka, C.]]
[[Category: Barinka C]]
[[Category: Lubkowski, J.]]
[[Category: Lubkowski J]]
[[Category: cc chemokine family]]
[[Category: ccl13]]
[[Category: chemotaxis]]
[[Category: cytokine]]
[[Category: inflammatory response]]
[[Category: mcp-4]]
[[Category: monocyte]]
[[Category: polymorphism]]
[[Category: pyrrolidone carboxylic acid]]
[[Category: secreted]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:04 2008''

Latest revision as of 11:37, 30 October 2024

Crystal Structure of Human Monocyte Chemoattractant Protein 4 (MCP-4/CCL13)Crystal Structure of Human Monocyte Chemoattractant Protein 4 (MCP-4/CCL13)

Structural highlights

2ra4 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CCL13_HUMAN Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Monocyte chemoattractant proteins (MCPs) belong to the CC chemokine family and are involved in many (patho)physiological processes characterized by mononuclear cell infiltration, including tissue remodeling, atherosclerosis and cancer metastasis. Here, the crystal structure of human monocyte chemoattractant protein 4 (MCP-4) refined at 1.70 A resolution is reported with crystallographic values R = 0.180 and R free = 0.212. The overall MCP-4 fold reveals the typical tertiary features of the CC chemokine family. A central three-stranded antiparallel beta-sheet is C-terminally flanked by an overlaying alpha-helix, while the N-terminal part of the molecule forms an extended loop that is anchored to the rest of the molecule via two disulfide bridges, Cys11-Cys35 and Cys12-Cys51. The crystal packing suggests the existence of MCP-4 dimers with a dimerization interface similar to those previously reported for the X-ray structures of MCP-1 and MCP-2.

Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13).,Barinka C, Prahl A, Lubkowski J Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):273-8. Epub 2008, Feb 20. PMID:18323622[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Barinka C, Prahl A, Lubkowski J. Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13). Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):273-8. Epub 2008, Feb 20. PMID:18323622 doi:10.1107/S0907444907066164

2ra4, resolution 1.70Å

Drag the structure with the mouse to rotate

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