3bs5: Difference between revisions

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-{{Seed}}
-[[Image:3bs5.png|left|200px]]
-<!--
+==Crystal Structure of hCNK2-SAM/dHYP-SAM Complex==
-The line below this paragraph, containing "STRUCTURE_3bs5", creates the "Structure Box" on the page.
+<StructureSection load='3bs5' size='340' side='right'caption='[[3bs5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3bs5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BS5 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-{{STRUCTURE_3bs5|  PDB=3bs5  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs5 OCA], [https://pdbe.org/3bs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bs5 RCSB], [https://www.ebi.ac.uk/pdbsum/3bs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AVE_DROME AVE_DROME] Required for normal photoreceptor differentiation between Ras and Raf for EGFR signaling in the eye and for mitogen-activated protein kinase phosphorylation. Probably acts together with Cnk to promote Raf activation, perhaps by recruiting an activating kinase.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bs5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bs5 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+RAF kinase functions in the mitogen-activated protein kinase (MAPK) pathway to transmit growth signals to the downstream kinases MEK and ERK. Activation of RAF catalytic activity is facilitated by a regulatory complex comprising the proteins CNK (Connector enhancer of KSR), HYP (Hyphen), and KSR (Kinase Suppressor of Ras). The sterile alpha-motif (SAM) domain found in both CNK and HYP plays an essential role in complex formation. Here, we have determined the x-ray crystal structure of the SAM domain of CNK in complex with the SAM domain of HYP. The structure reveals a single-junction SAM domain dimer of 1:1 stoichiometry in which the binding mode is a variation of polymeric SAM domain interactions. Through in vitro and in vivo mutational analyses, we show that the specific mode of dimerization revealed by the crystal structure is essential for RAF signaling and facilitates the recruitment of KSR to form the CNK/HYP/KSR regulatory complex. We present two docking-site models to account for how SAM domain dimerization might influence the formation of a higher-order CNK/HYP/KSR complex.
-===Crystal Structure of hCNK2-SAM/dHYP-SAM Complex===
+CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling.,Rajakulendran T, Sahmi M, Kurinov I, Tyers M, Therrien M, Sicheri F Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2836-41. Epub 2008 Feb 19. PMID:18287031<ref>PMID:18287031</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_18287031}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3bs5" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 18287031 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18287031}}
+__TOC__
+</StructureSection>
-==About this Structure==
-BS5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS5 OCA].
-==Reference==
-CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling., Rajakulendran T, Sahmi M, Kurinov I, Tyers M, Therrien M, Sicheri F, Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2836-41. Epub 2008 Feb 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18287031 18287031]
 [[Category: Drosophila melanogaster]]
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Ceccarelli, D F.]]
+[[Category: Ceccarelli DF]]
-[[Category: Kurinov, I.]]
+[[Category: Kurinov I]]
-[[Category: Rajakulendran, T.]]
+[[Category: Rajakulendran T]]
-[[Category: Sicheri, F.]]
+[[Category: Sicheri F]]
-[[Category: Alternative splicing]]
-[[Category: Coiled coil]]
-[[Category: Cytoplasm]]
-[[Category: Membrane]]
-[[Category: Phosphoprotein]]
-[[Category: Polymorphism]]
-[[Category: Sam domain]]
-[[Category: Sam domain complex]]
-[[Category: Sam domain dimer]]
-[[Category: Sensory transduction]]
-[[Category: Signaling protein/membrane protein complex]]
-[[Category: Sterile alpha motif]]
-[[Category: Vision]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 20 11:24:42 2008''