2qk7: Difference between revisions

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-{{Seed}}
-[[Image:2qk7.png|left|200px]]
-<!--
+==A covalent S-F heterodimer of staphylococcal gamma-hemolysin==
-The line below this paragraph, containing "STRUCTURE_2qk7", creates the "Structure Box" on the page.
+<StructureSection load='2qk7' size='340' side='right'caption='[[2qk7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2qk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MW2 Staphylococcus aureus subsp. aureus MW2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QK7 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qk7 OCA], [https://pdbe.org/2qk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qk7 RCSB], [https://www.ebi.ac.uk/pdbsum/2qk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qk7 ProSAT]</span></td></tr>
-{{STRUCTURE_2qk7|  PDB=2qk7  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HLGA_STAAU HLGA_STAAU] Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qk/2qk7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qk7 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Staphylococcal leucotoxins, leucocidins, and gamma-hemolysins are bicomponent beta-barrel pore-forming toxins (beta-PFTs). Their production is associated with several clinical diseases. They have cytotoxic activity due to the synergistic action of a class S component and a class F component, which are secreted as water-soluble monomers and form hetero-oligomeric transmembrane pores, causing the lysis of susceptible cells. Structural information is currently available for the monomeric S and F proteins and the homoheptamer formed by the related alpha-hemolysin. These structures illustrate the start and end points in the mechanistic framework of beta-PFT assembly. Only limited structural data exist for the intermediate stages, including hetero-oligomeric complexes of leucotoxins. We investigated the protein-protein interactions responsible for maintaining the final bipartite molecular architecture and describe here the high-resolution crystal structure and low-resolution solution structure of a site-specific cross-linked heterodimer of gamma-hemolysin (HlgA T28C-HlgB N156C), which were solved by X-ray crystallography and small angle X-ray scattering, respectively. These structures reveal a molecular plasticity of beta-PFTs, which may facilitate the transition from membrane-bound monomers to heterodimers. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
-===A covalent S-F heterodimer of staphylococcal gamma-hemolysin===
+A covalent S-F heterodimer of leucotoxin reveals molecular plasticity of beta-barrel pore-forming toxins.,Roblin P, Guillet V, Joubert O, Keller D, Erard M, Maveyraud L, Prevost G, Mourey L Proteins. 2008 Jan 23;71(1):485-496. PMID:18214982<ref>PMID:18214982</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2qk7" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18214982}}, adds the Publication Abstract to the page
+*[[Hemolysin 3D structures|Hemolysin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18214982 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18214982}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-QK7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QK7 OCA].
+[[Category: Staphylococcus aureus subsp. aureus MW2]]
+[[Category: Guillet V]]
-==Reference==
+[[Category: Maveyraud L]]
-A covalent S-F heterodimer of leucotoxin reveals molecular plasticity of beta-barrel pore-forming toxins., Roblin P, Guillet V, Joubert O, Keller D, Erard M, Maveyraud L, Prevost G, Mourey L, Proteins. 2008 Jan 23;71(1):485-496. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18214982 18214982]
+[[Category: Mourey L]]
+[[Category: Roblin P]]
-Engineered covalent leucotoxin heterodimers form functional pores: insights into S-F interactions., Joubert O, Viero G, Keller D, Martinez E, Colin DA, Monteil H, Mourey L, Dalla Serra M, Prevost G, Biochem J. 2006 Jun 1;396(2):381-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16494579 16494579]
-[[Category: Protein complex]]
-[[Category: Staphylococcus aureus]]
-[[Category: Guillet, V.]]
-[[Category: Maveyraud, L.]]
-[[Category: Mourey, L.]]
-[[Category: Roblin, P.]]
-[[Category: Beta-barrel]]
-[[Category: Covalent complex]]
-[[Category: Cytolysis]]
-[[Category: Hemolysis]]
-[[Category: Molecular plasticity]]
-[[Category: Pore-forming toxin]]
-[[Category: Protein-protein interaction]]
-[[Category: Secreted]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:54:10 2008''