2gn3: Difference between revisions

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-[[Image:2gn3.gif|left|200px]]
- {{Structure
+==Metal-free (apo-PAL) in complex with alpha-D-Met-Man==
-|PDB= 2gn3 |SIZE=350|CAPTION= <scene name='initialview01'>2gn3</scene>, resolution 1.97&Aring;
+<StructureSection load='2gn3' size='340' side='right'caption='[[2gn3]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+<table><tr><td colspan='2'>[[2gn3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GN3 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gn3 OCA], [https://pdbe.org/2gn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gn3 RCSB], [https://www.ebi.ac.uk/pdbsum/2gn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gn3 ProSAT]</span></td></tr>
-|RELATEDENTRY=[[1ukg|1UKG]], [[2gme|2GME]], [[2gmm|2GMM]], [[2gmp|2GMP]], [[2gnb|2GNB]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gn3 OCA], [http://www.ebi.ac.uk/pdbsum/2gn3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gn3 RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/Q8GSD2_PTEAG Q8GSD2_PTEAG]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/2gn3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gn3 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype.
-'''Metal-free (apo-PAL) in complex with alpha-D-Met-Man'''
+Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.,Garcia-Pino A, Buts L, Wyns L, Loris R J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21. PMID:16824540<ref>PMID:16824540</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2gn3" style="background-color:#fffaf0;"></div>
-==Overview==
+==See Also==
-The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype.
+*[[Pal|Pal]]
+== References ==
-==About this Structure==
+<references/>
-GN3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GN3 OCA].
+__TOC__
+</StructureSection>
-==Reference==
+[[Category: Large Structures]]
-Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin., Garcia-Pino A, Buts L, Wyns L, Loris R, J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16824540 16824540]
 [[Category: Pterocarpus angolensis]]
-[[Category: Single protein]]
+[[Category: Buts L]]
-[[Category: Buts, L.]]
+[[Category: Garcia-Pino A]]
-[[Category: Garcia-Pino, A.]]
+[[Category: Loris R]]
-[[Category: Loris, R.]]
+[[Category: Wyns L]]
-[[Category: Wyns, L.]]
-[[Category: beta sandwich]]
-[[Category: legume lectin]]
-[[Category: mannose]]
-[[Category: metal-free lectin]]
-[[Category: sugar complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:18:51 2008''