2g6y: Difference between revisions

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-{{Seed}}
-[[Image:2g6y.png|left|200px]]
-<!--
+==Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata==
-The line below this paragraph, containing "STRUCTURE_2g6y", creates the "Structure Box" on the page.
+<StructureSection load='2g6y' size='340' side='right'caption='[[2g6y]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2g6y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pontellina_plumata Pontellina plumata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G6Y FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
-{{STRUCTURE_2g6y|  PDB=2g6y  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g6y OCA], [https://pdbe.org/2g6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g6y RCSB], [https://www.ebi.ac.uk/pdbsum/2g6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g6y ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q6WV12_9MAXI Q6WV12_9MAXI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/2g6y_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g6y ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states. However, we are still far from a complete understanding of the origin, functions and evolution of the GFP family. Novel proteins of the family were recently cloned from evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein and solved its high-resolution crystal structure. It was found that the protein beta-barrel contains a pore, leading to the chromophore. Using site-directed mutagenesis, we showed that this feature is critical for the fast maturation of the chromophore.
-===Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata===
+Structural basis for the fast maturation of Arthropoda green fluorescent protein.,Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM EMBO Rep. 2006 Oct;7(10):1006-12. Epub 2006 Aug 25. PMID:16936637<ref>PMID:16936637</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2g6y" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16936637}}, adds the Publication Abstract to the page
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16936637 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16936637}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-G6Y is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Pontellina_plumata Pontellina plumata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G6Y OCA].
-==Reference==
-<ref group="xtra">PMID:16936637</ref><references group="xtra"/>
 [[Category: Pontellina plumata]]
-[[Category: Chudakov, D M.]]
+[[Category: Chudakov DM]]
-[[Category: Evdokimov, A G.]]
+[[Category: Evdokimov AG]]
-[[Category: Pokross, M E.]]
+[[Category: Pokross ME]]
-[[Category: Beta-can]]
-[[Category: Green fluorescent protein]]
-[[Category: Natural chromophore]]
-[[Category: Rapid maturation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:57:31 2009''