2h4c: Difference between revisions

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-[[Image:2h4c.jpg|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_2h4c", creates the "Structure Box" on the page.
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-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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-{{STRUCTURE_2h4c|  PDB=2h4c  |  SCENE=  }}
-'''Structure of Daboiatoxin (heterodimeric PLA2 venom)'''
+==Structure of Daboiatoxin (heterodimeric PLA2 venom)==
+<StructureSection load='2h4c' size='340' side='right'caption='[[2h4c]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2h4c]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Daboia_siamensis Daboia siamensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H4C FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h4c OCA], [https://pdbe.org/2h4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h4c RCSB], [https://www.ebi.ac.uk/pdbsum/2h4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h4c ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PA2AA_DABSI PA2AA_DABSI] Heterodimer (A and B chains): phospholipase A2 that acts as a presynaptic neurotoxin and shows a PLA2 activity of 1377 umol/min/mg. In vivo, induces edema and produces neurotoxic symptoms in mice. Also exhibits indirect hemolysis, a strong myonecrotic activity and cytotoxicity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.  Monomer: Snake venom phospholipase A2 (PLA2) that shows a PLA2 activity of 578 umol/min/mg.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/2h4c_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h4c ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Russell's viper (Vipera russelli, also known as Daboia russelli) is one of the major causes of fatal snakebites. To date, five Daboia russelli subspecies have been recognized. Daboiatoxin (DbTx) is the main lethal phospholipase A(2) (PLA(2)) toxin in the venom of D. russelli siamensis (Myanmar viper) and has strong neurotoxic, myotoxic and cytotoxic activities. DbTx and its homologous neurotoxins viperotoxin F from D. russelli formosensis (Taiwan viper) and vipoxin from the Bulgarian sand viper V. ammodytes meridionalis consist of complexes between a nontoxic acidic PLA(2) protein and an enzymatically active basic PLA(2). DbTx and viperotoxin F are presynaptic toxins, while vipoxin is postsynaptic. The two chains of DbTx have been separated and their PLA(2) enzymatic activity has been measured using the secretory PLA(2) assay kit. The enzymatic activity of DbTx chain B is reduced by 30% of its original activity by chain A in a unimolar ratio, thus indicating that DbTx chain A acts as an inhibitor. The lethal activity of the two chains has also been studied in male albino mice and chain A is less lethal than chain B. The crystal structure of DbTx has also been determined and its structural details are compared with those of the two homologues. Furthermore, an attempt is made to correlate the sequence and structural determinants of these toxins with their enzymatic activities and their pharmacological effects.
-==Overview==
+Structural and pharmacological comparison of daboiatoxin from Daboia russelli siamensis with viperotoxin F and vipoxin from other vipers.,Gopalan G, Thwin MM, Gopalakrishnakone P, Swaminathan K Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):722-9. Epub 2007, May 15. PMID:17505111<ref>PMID:17505111</ref>
-Russell's viper (Vipera russelli, also known as Daboia russelli) is one of the major causes of fatal snakebites. To date, five Daboia russelli subspecies have been recognized. Daboiatoxin (DbTx) is the main lethal phospholipase A(2) (PLA(2)) toxin in the venom of D. russelli siamensis (Myanmar viper) and has strong neurotoxic, myotoxic and cytotoxic activities. DbTx and its homologous neurotoxins viperotoxin F from D. russelli formosensis (Taiwan viper) and vipoxin from the Bulgarian sand viper V. ammodytes meridionalis consist of complexes between a nontoxic acidic PLA(2) protein and an enzymatically active basic PLA(2). DbTx and viperotoxin F are presynaptic toxins, while vipoxin is postsynaptic. The two chains of DbTx have been separated and their PLA(2) enzymatic activity has been measured using the secretory PLA(2) assay kit. The enzymatic activity of DbTx chain B is reduced by 30% of its original activity by chain A in a unimolar ratio, thus indicating that DbTx chain A acts as an inhibitor. The lethal activity of the two chains has also been studied in male albino mice and chain A is less lethal than chain B. The crystal structure of DbTx has also been determined and its structural details are compared with those of the two homologues. Furthermore, an attempt is made to correlate the sequence and structural determinants of these toxins with their enzymatic activities and their pharmacological effects.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-H4C is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Daboia_russellii_siamensis Daboia russellii siamensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H4C OCA].
+</div>
+<div class="pdbe-citations 2h4c" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Structural and pharmacological comparison of daboiatoxin from Daboia russelli siamensis with viperotoxin F and vipoxin from other vipers., Gopalan G, Thwin MM, Gopalakrishnakone P, Swaminathan K, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):722-9. Epub 2007, May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17505111 17505111]
+*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
-[[Category: Daboia russellii siamensis]]
+== References ==
-[[Category: Protein complex]]
+<references/>
-[[Category: Gopalakrishnakone, P.]]
+__TOC__
-[[Category: Gopalan, G.]]
+</StructureSection>
-[[Category: Swaminathan, K.]]
+[[Category: Daboia siamensis]]
-[[Category: Thwin, M M.]]
+[[Category: Large Structures]]
-[[Category: Basic pla2]]
+[[Category: Gopalakrishnakone P]]
-[[Category: Heterodimer]]
+[[Category: Gopalan G]]
-[[Category: Non-inhibitor acidic pla2]]
+[[Category: Swaminathan K]]
-[[Category: Phospholipase a2]]
+[[Category: Thwin MM]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:51:10 2008''