2nx7: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2nx7.jpg|left|200px]]<br /><applet load="2nx7" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2nx7" />
-'''Structure of NOWA cysteine rich domain 8'''<br />
-==Overview==
+==Structure of NOWA cysteine rich domain 8==
-The nematocyst wall of cnidarians is a unique biomaterial that withstands, extreme osmotic pressures, allowing an ultrafast discharge of the, nematocyst capsules. Assembly of the highly robust nematocyst wall is, achieved by covalent linkage of cysteine-rich domains (CRDs) from two main, protein components, minicollagens and nematocyst outer wall antigen, (NOWA). The bipolar minicollagens have different disulfide patterns and, topologies in their N and C-terminal CRDs. The functional significance of, this polarity has been elusive. Here, we show by NMR structural analysis, that all representative cysteine-rich domains of NOWA are structurally, related to N-terminal minicollagen domains. Natural sequence insertions in, NOWA CRDs have very little effect on the tightly knit domain structures, nor do they preclude the efficient folding to a single native, conformation. The different folds in NOWA CRDs and the atypical C-terminal, minicollagen domain on the other hand can be directly related to different, conformational preferences in the reduced states. Ultrastructural analysis, in conjunction with aggregation studies argues for an association between, the similar NOWA and N-terminal minicollagen domains in early stages of, the nematocyst wall assembly, which is followed by the controlled, association between the unusual structures of C-terminal minicollagen, domains.
+<StructureSection load='2nx7' size='340' side='right'caption='[[2nx7]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2nx7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hydra_vulgaris Hydra vulgaris]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NX7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nx7 OCA], [https://pdbe.org/2nx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nx7 RCSB], [https://www.ebi.ac.uk/pdbsum/2nx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nx7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8IT70_HYDVU Q8IT70_HYDVU]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The nematocyst wall of cnidarians is a unique biomaterial that withstands extreme osmotic pressures, allowing an ultrafast discharge of the nematocyst capsules. Assembly of the highly robust nematocyst wall is achieved by covalent linkage of cysteine-rich domains (CRDs) from two main protein components, minicollagens and nematocyst outer wall antigen (NOWA). The bipolar minicollagens have different disulfide patterns and topologies in their N and C-terminal CRDs. The functional significance of this polarity has been elusive. Here, we show by NMR structural analysis that all representative cysteine-rich domains of NOWA are structurally related to N-terminal minicollagen domains. Natural sequence insertions in NOWA CRDs have very little effect on the tightly knit domain structures, nor do they preclude the efficient folding to a single native conformation. The different folds in NOWA CRDs and the atypical C-terminal minicollagen domain on the other hand can be directly related to different conformational preferences in the reduced states. Ultrastructural analysis in conjunction with aggregation studies argues for an association between the similar NOWA and N-terminal minicollagen domains in early stages of the nematocyst wall assembly, which is followed by the controlled association between the unusual structures of C-terminal minicollagen domains.
-==About this Structure==
+Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall.,Meier S, Jensen PR, Adamczyk P, Bachinger HP, Holstein TW, Engel J, Ozbek S, Grzesiek S J Mol Biol. 2007 May 4;368(3):718-28. Epub 2007 Feb 24. PMID:17362991<ref>PMID:17362991</ref>
-NX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hydra_vulgaris Hydra vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NX7 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Sequence-Structure and Structure-Function Analysis in Cysteine-rich Domains Forming the Ultrastable Nematocyst Wall., Meier S, Jensen PR, Adamczyk P, Bachinger HP, Holstein TW, Engel J, Ozbek S, Grzesiek S, J Mol Biol. 2007 May 4;368(3):718-28. Epub 2007 Feb 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17362991 17362991]
+</div>
+<div class="pdbe-citations 2nx7" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Hydra vulgaris]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Adamczyk, P.]]
+[[Category: Adamczyk P]]
-[[Category: Bachinger, H.P.]]
+[[Category: Bachinger HP]]
-[[Category: Engel, J.]]
+[[Category: Engel J]]
-[[Category: Grzesiek, S.]]
+[[Category: Grzesiek S]]
-[[Category: Holstein, T.W.]]
+[[Category: Holstein TW]]
-[[Category: Jensen, P.R.]]
+[[Category: Jensen PR]]
-[[Category: Meier, S.]]
+[[Category: Meier S]]
-[[Category: Ozbek, S.]]
+[[Category: Ozbek S]]
-[[Category: cysteine rich]]
-[[Category: disulfide bonds]]
-[[Category: rich in turns]]
-[[Category: structural protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:18:23 2008''