2eh8: Difference between revisions

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-[[Image:2eh8.png|left|200px]]
-{{STRUCTURE_2eh8|  PDB=2eh8  |  SCENE=  }}
+==Crystal structure of the complex of humanized KR127 fab and PRES1 peptide epitope==
+<StructureSection load='2eh8' size='340' side='right'caption='[[2eh8]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2eh8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_B_virus Hepatitis B virus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EH8 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eh8 OCA], [https://pdbe.org/2eh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eh8 RCSB], [https://www.ebi.ac.uk/pdbsum/2eh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eh8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2EID8_HBV Q2EID8_HBV] The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid (By similarity).[SAAS:SAAS000349_004_055156]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/2eh8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eh8 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo. We present the crystal structures of HzKR127 Fab and its complex with a major epitope peptide. In the complex structure, the bound peptide forms a type IV beta-turn followed by 3(10) helical turn, the looped-out conformation of which provides a structural basis for broad neutralization. Upon peptide binding, the antibody undergoes a dramatic complementarity determining region H3 lid opening. To understand the structural implication of the virus neutralization, we carried out comprehensive alanine-scanning mutagenesis of all complementarity determining region residues in HzKR127 Fab. The functional mapping of the antigen-combining site demonstrates the specific roles of major binding determinants in antigen binding, contributing to the rational design for maximal humanization and affinity maturation of the antibody.
-===Crystal structure of the complex of humanized KR127 fab and PRES1 peptide epitope===
+Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism.,Chi SW, Maeng CY, Kim SJ, Oh MS, Ryu CJ, Kim SJ, Han KH, Hong HJ, Ryu SE Proc Natl Acad Sci U S A. 2007 May 29;104(22):9230-5. Epub 2007 May 17. PMID:17517649<ref>PMID:17517649</ref>
-{{ABSTRACT_PUBMED_17517649}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2eh8" style="background-color:#fffaf0;"></div>
-[[2eh8]] is a 3 chain structure of [[Antibody]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH8 OCA].
 ==See Also==
-*[[Antibody|Antibody]]
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
-==Reference==
+== References ==
-<ref group="xtra">PMID:017517649</ref><references group="xtra"/>
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Hepatitis B virus]]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Chi, S W.]]
+[[Category: Chi S-W]]
-[[Category: Hong, H J.]]
+[[Category: Hong HJ]]
-[[Category: Kim, S J.]]
+[[Category: Kim S-J]]
-[[Category: Maeng, C Y.]]
+[[Category: Maeng C-Y]]
-[[Category: Ryu, S E.]]
+[[Category: Ryu S-E]]
-[[Category: Hepatitis b virus]]
-[[Category: Humanized antibody]]
-[[Category: Immune system]]
-[[Category: Monoclonal antibody]]
-[[Category: Neutralization]]
-[[Category: Pres1]]