1i8e: Difference between revisions
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==NMR ENSEMBLE OF ION-SELECTIVE LIGAND A22 FOR PLATELET INTEGRIN ALPHAIIB-BETA3== | ==NMR ENSEMBLE OF ION-SELECTIVE LIGAND A22 FOR PLATELET INTEGRIN ALPHAIIB-BETA3== | ||
<StructureSection load='1i8e' size='340' side='right' caption='[[1i8e | <StructureSection load='1i8e' size='340' side='right'caption='[[1i8e]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1i8e]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8E OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[1i8e]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8E FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8e OCA], [https://pdbe.org/1i8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8e RCSB], [https://www.ebi.ac.uk/pdbsum/1i8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 21: | Line 21: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Assa-Munt | [[Category: Large Structures]] | ||
[[Category: | [[Category: Assa-Munt N]] | ||
[[Category: | [[Category: Jia X]] | ||
[[Category: Parra-Gessert | [[Category: Le Calvez H]] | ||
[[Category: Preece | [[Category: Parra-Gessert L]] | ||
[[Category: Smith | [[Category: Preece NE]] | ||
[[Category: Smith JW]] | |||
Latest revision as of 09:44, 30 October 2024
NMR ENSEMBLE OF ION-SELECTIVE LIGAND A22 FOR PLATELET INTEGRIN ALPHAIIB-BETA3NMR ENSEMBLE OF ION-SELECTIVE LIGAND A22 FOR PLATELET INTEGRIN ALPHAIIB-BETA3
Structural highlights
Publication Abstract from PubMedIntegrins contain a number of divalent cation binding sites that control ligand binding affinity. Ions such as Ca(2+) and Mg(2+) bind to distinct sites on integrin and can have opposing effects on ligand binding. These effects are presumably brought about by alterations of the shape of the ligand binding pocket. To gain insight into the nature of these structural differences, we probed the integrin ligand binding site with an RGD-based library of unparalleled complexity. A cysteine-constrained phage library containing six random amino acids and the RGD motif present in seven different registers was used to select for ligands that exhibit ion-selective binding to integrin alpha(IIb)beta(3). The library was used to select for peptides that bind to the integrin alpha(IIb)beta(3) preferentially in Ca(2+) versus Mg(2+). Peptides were identified which bound selectively in each ion. The Ca(2+)-selective peptides had a range of sequences, with the only obvious consensus involving a motif that had four cysteine residues bonded in a 1,4:2,3 arrangement. Interestingly though, the Mg(2+)-selective peptides exhibited a well defined consensus motif containing Cys-X-aromatic-L/G-R-G-D-hydrophobic-R-R/K-Cys. As a first step toward understanding the structural basis for this selectivity, solution NMR structures were obtained for representatives of both sets of peptides. All peptides formed turns, with the RGD motif at the apex. The Mg(2+)-selected peptides contained a unique basic patch that protrudes from the base of the turn. Selection and structure of ion-selective ligands for platelet integrin alpha IIb(beta) 3.,Smith JW, Le Calvez H, Parra-Gessert L, Preece NE, Jia X, Assa-Munt N J Biol Chem. 2002 Mar 22;277(12):10298-305. Epub 2001 Dec 17. PMID:11748219[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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