1tih: Difference between revisions
New page: left|200px<br /><applet load="1tih" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tih" /> '''TRYPSIN INHIBITOR (T1) FROM NICOTIANA ALATA'... |
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== | ==TRYPSIN INHIBITOR (T1) FROM NICOTIANA ALATA== | ||
The three-dimensional structures of a series of 6-kDa trypsin inhibitors | <StructureSection load='1tih' size='340' side='right'caption='[[1tih]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1tih]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TIH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tih OCA], [https://pdbe.org/1tih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tih RCSB], [https://www.ebi.ac.uk/pdbsum/1tih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tih ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q40378_NICAL Q40378_NICAL] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ti/1tih_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tih ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The three-dimensional structures of a series of 6-kDa trypsin inhibitors isolated from the stigma of the ornamental tobacco Nicotiana alata have been determined by 1H NMR spectroscopy combined with simulated annealing calculations. The proteins, T1-T4, are proteolytically cleaved from a 40.3-kDa precursor protein, NA-proPI, together with a chymotrypsin inhibitor, C1, the structure of which was reported recently [Nielsen, K.J., Heath, R.L., Anderson, M.A., & Craik, D.J. (1994) J. Mol. Biol. 242, 231-243]. Each of the proteinase inhibitors comprises 53 amino acids, including 8 cysteine residues which are linked to form 4 disulfide bridges. The proteins have a high degree of sequence identity and differ mainly in residues around the putative reactive sites. The structure of T1 was determined using a set of 533 interproton distance restraints derived from NOESY spectra, combined with 33 dihedral restraints derived from 3JNH-H alpha coupling constants and 16 hydrogen bonds. The structures of the remaining inhibitors (T2-T4) were deduced to be almost identical to T1, on the basis of their similar chemical shifts and 2D spectra. The current study demonstrates that the structures of the trypsin inhibitors (T1-T4) are similar to that previously found for the chymotrypsin inhibitor, C1. Despite differences in sequence, there is conservation in backbone geometry between the reactive site loops of the two classes of inhibitors. From this, it is clear that the nature of the side chain on the primary binding residue, rather than the backbone fold, is the main determinant of the enzyme specificities of these proteinase inhibitors. | |||
Structures of a series of 6-kDa trypsin inhibitors isolated from the stigma of Nicotiana alata.,Nielsen KJ, Heath RL, Anderson MA, Craik DJ Biochemistry. 1995 Nov 7;34(44):14304-11. PMID:7578034<ref>PMID:7578034</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1tih" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Nicotiana alata]] | [[Category: Nicotiana alata]] | ||
[[Category: Anderson MA]] | |||
[[Category: Anderson | [[Category: Craik DJ]] | ||
[[Category: Craik | [[Category: Heath RL]] | ||
[[Category: Heath | [[Category: Nielsen KJ]] | ||
[[Category: Nielsen | |||
