1tg7: Difference between revisions
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==Native structure of beta-galactosidase from Penicillium sp.== | ==Native structure of beta-galactosidase from Penicillium sp.== | ||
<StructureSection load='1tg7' size='340' side='right' caption='[[1tg7]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='1tg7' size='340' side='right'caption='[[1tg7]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1tg7]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1tg7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_sp. Penicillium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TG7 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tg7 OCA], [https://pdbe.org/1tg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tg7 RCSB], [https://www.ebi.ac.uk/pdbsum/1tg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tg7 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/BGALA_PENSQ BGALA_PENSQ] Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Has high in vitro transglycosylation activity with p-nitrophenyl-beta-D-galactopyranoside, methyl-beta-D-galactopyranoside or lactose as a donor and galactose as an acceptor.<ref>PMID:11053867</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tg7_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tg7_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1tg7" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1tg7" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Galactosidase 3D structures|Galactosidase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Penicillium sp]] | [[Category: Penicillium sp]] | ||
[[Category: Adamska | [[Category: Adamska M]] | ||
[[Category: Arand | [[Category: Arand M]] | ||
[[Category: Eneyskaya | [[Category: Eneyskaya EV]] | ||
[[Category: Garratt | [[Category: Garratt RC]] | ||
[[Category: Golubev | [[Category: Golubev AM]] | ||
[[Category: Kulminskaya | [[Category: Kulminskaya AA]] | ||
[[Category: Nagem | [[Category: Nagem RAP]] | ||
[[Category: Neustroev | [[Category: Neustroev KN]] | ||
[[Category: Polikarpov | [[Category: Polikarpov I]] | ||
[[Category: Rojas | [[Category: Rojas AL]] | ||
Latest revision as of 10:28, 30 October 2024
Native structure of beta-galactosidase from Penicillium sp.Native structure of beta-galactosidase from Penicillium sp.
Structural highlights
FunctionBGALA_PENSQ Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Has high in vitro transglycosylation activity with p-nitrophenyl-beta-D-galactopyranoside, methyl-beta-D-galactopyranoside or lactose as a donor and galactose as an acceptor.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedBeta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. Penicillium sp. beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date. Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose.,Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I J Mol Biol. 2004 Nov 5;343(5):1281-92. PMID:15491613[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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