1rj1: Difference between revisions

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-{{Seed}}
-[[Image:1rj1.png|left|200px]]
-<!--
+==Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco==
-The line below this paragraph, containing "STRUCTURE_1rj1", creates the "Structure Box" on the page.
+<StructureSection load='1rj1' size='340' side='right'caption='[[1rj1]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1rj1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RJ1 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rj1 OCA], [https://pdbe.org/1rj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rj1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rj1 ProSAT]</span></td></tr>
-{{STRUCTURE_1rj1|  PDB=1rj1  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O49908_TOBAC O49908_TOBAC]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/1rj1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rj1 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Plant invertases are sucrolytic enzymes essential for plant metabolism and development. Enzyme activity is regulated on a posttranslational level via inhibitory proteins, referred to as invertase inhibitors. Ectopic expression of invertase inhibitors in crop plants has high biotechnological potential. However, little biochemical and up to now no detailed structural information is available about this class of plant regulatory proteins. Here, we present the crystal structure of the cell wall-associated invertase inhibitor Nt-CIF from tobacco at a resolution of 1.87A. The structural model reveals an asymmetric four-helix bundle with an uncommon N-terminal extension that appears to be critical for the structural integrity of the protein. Structure analysis of a second crystal form grown in the presence of CdCl(2) reveals two metal binding sites. Nt-CIF is highly thermostable and retains full inhibitory activity after cooling to ambient temperatures. The structure of Nt-CIF provides the first three-dimensional information source for the posttranslational regulation of plant invertases. Based on the recently discovered sequence homology between inhibitors of invertases and pectin methylesterases, our structural model is likely to represent a scaffold also used for the regulation of the latter enzymes, which do not share sequence similarity with invertases. Thus, our structural model sets the 3D-stage for the investigation of posttranslational regulation of invertases as well as pectin methylesterases.
-===Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco===
+The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension.,Hothorn M, D'Angelo I, Marquez JA, Greiner S, Scheffzek K J Mol Biol. 2004 Jan 23;335(4):987-95. PMID:14698293<ref>PMID:14698293</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_14698293}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1rj1" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 14698293 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_14698293}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-RJ1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ1 OCA].
-==Reference==
-<ref group="xtra">PMID:14698293</ref><references group="xtra"/>
 [[Category: Nicotiana tabacum]]
-[[Category: Angelo, I D.]]
+[[Category: D'Angelo I]]
-[[Category: Greiner, S.]]
+[[Category: Greiner S]]
-[[Category: Hothorn, M.]]
+[[Category: Hothorn M]]
-[[Category: Marquez, J A.]]
+[[Category: Marquez JA]]
-[[Category: Scheffzek, K.]]
+[[Category: Scheffzek K]]
-[[Category: Four-helix bundle]]
-[[Category: Helical hairpin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:16:25 2009''