1n4i: Difference between revisions

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-{{Seed}}
-[[Image:1n4i.png|left|200px]]
-<!--
+==Solution structure of spruce budworm antifreeze protein at 5 degrees celsius==
-The line below this paragraph, containing "STRUCTURE_1n4i", creates the "Structure Box" on the page.
+<StructureSection load='1n4i' size='340' side='right'caption='[[1n4i]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1n4i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4I FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 25 models</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4i OCA], [https://pdbe.org/1n4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4i RCSB], [https://www.ebi.ac.uk/pdbsum/1n4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4i ProSAT]</span></td></tr>
-{{STRUCTURE_1n4i|  PDB=1n4i  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9GTP0_CHOFU Q9GTP0_CHOFU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4i_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4i ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Antifreeze proteins (AFPs) prevent the growth of ice, and are used by some organisms that live in sub-zero environments for protection against freezing. All AFPs are thought to function by an adsorption inhibition process. In order to elucidate the ice-binding mechanism, the structures of several AFPs have been determined, and have been shown to consist of different folds. Recently, the first structures of the highly active insect AFPs have been characterized. These proteins have a beta-helix structure, which adds yet another fold to the AFP family. The 90-residue spruce budworm (Choristoneura fumiferana) AFP consists of a beta-helix with 15 residues per coil. The structure contains two ranks of aligned threonine residues (known as the TXT motif), which were shown by mutagenesis experiments to be located in the ice-binding face. In our previous NMR study of this AFP at 30 degrees C, we found that the TXT face was not optimally defined because of the broadening of NMR resonances potentially due to weak oligomerization. We present here a structure of spruce budworm AFP determined at 5 degrees C, where this broadening is reduced. In addition, the 1H-15N NMR dynamics of the protein were examined at 30 degrees C and 5 degrees C. The results show that the spruce budworm AFP is more structured at 5 degrees C, and support the general observation that AFPs become more rigid as the temperature is lowered.
-===Solution structure of spruce budworm antifreeze protein at 5 degrees celsius===
+Spruce budworm antifreeze protein: changes in structure and dynamics at low temperature.,Graether SP, Gagne SM, Spyracopoulos L, Jia Z, Davies PL, Sykes BD J Mol Biol. 2003 Apr 11;327(5):1155-68. PMID:12662938<ref>PMID:12662938</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1n4i" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_12662938}}, adds the Publication Abstract to the page
+*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 12662938 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12662938}}
+__TOC__
+</StructureSection>
-==About this Structure==
-N4I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4I OCA].
-==Reference==
-Spruce budworm antifreeze protein: changes in structure and dynamics at low temperature., Graether SP, Gagne SM, Spyracopoulos L, Jia Z, Davies PL, Sykes BD, J Mol Biol. 2003 Apr 11;327(5):1155-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12662938 12662938]
 [[Category: Choristoneura fumiferana]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Davies, P L.]]
+[[Category: Davies PL]]
-[[Category: Gagne, S M.]]
+[[Category: Gagne SM]]
-[[Category: Graether, S P.]]
+[[Category: Graether SP]]
-[[Category: Jia, Z.]]
+[[Category: Jia Z]]
-[[Category: Spyracopoulos, L.]]
+[[Category: Spyracopoulos L]]
-[[Category: Sykes, B D.]]
+[[Category: Sykes BD]]
-[[Category: Antifreeze protein]]
-[[Category: Beta-helix]]
-[[Category: Ice]]
-[[Category: Insect]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:18:13 2008''