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[[Image:1qz0.gif|left|200px]]


{{Structure
==Crystal Structure of the Yersinia Pestis Phosphatase YopH in Complex with a Phosphotyrosyl Mimetic-Containing Hexapeptide==
|PDB= 1qz0 |SIZE=350|CAPTION= <scene name='initialview01'>1qz0</scene>, resolution 1.5&Aring;
<StructureSection load='1qz0' size='340' side='right'caption='[[1qz0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CLE:LEUCINE+AMIDE'>CLE</scene>, <scene name='pdbligand=FTY:DEOXY-DIFLUOROMETHELENE-PHOSPHOTYROSINE'>FTY</scene>
<table><tr><td colspan='2'>[[1qz0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZ0 FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FTY:DEOXY-DIFLUOROMETHELENE-PHOSPHOTYROSINE'>FTY</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qz0 OCA], [https://pdbe.org/1qz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qz0 RCSB], [https://www.ebi.ac.uk/pdbsum/1qz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qz0 ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qz0 OCA], [http://www.ebi.ac.uk/pdbsum/1qz0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qz0 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/O68720_YERPE O68720_YERPE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qz0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qz0 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Yersinia pestis, the causative agent of bubonic plague, secretes a eukaryotic-like protein tyrosine phosphatase (PTPase) termed Yersinia outer protein H (YopH) that is essential for virulence. We have determined, for the first time, the crystal structure of the YopH PTPase domain in complex with a nonhydrolyzable substrate analogue, the hexapeptide mimetic Ac-DADE-F(2)Pmp-L-NH(2). As anticipated, the mode of ligand binding in the active site is similar to the way in which the corresponding phosphohexapeptide binds to the structurally homologous human PTP1B. Unexpectedly, however, the crystal structure also revealed a second substrate-binding site in YopH that is not present in PTP1B. The mode of binding and structural conformation of the hexapeptide analogue is quite different in the two sites. Although the biological function of the second substrate-binding site remains to be investigated, the structure of a substrate analogue in the active site of Y. pestis YopH opens the door for the structure-based design and optimization of therapeutic countermeasures to combat this potential agent of bioterrorism.


'''Crystal Structure of the Yersinia Pestis Phosphatase YopH in Complex with a Phosphotyrosyl Mimetic-Containing Hexapeptide'''
High-resolution structure of the Yersinia pestis protein tyrosine phosphatase YopH in complex with a phosphotyrosyl mimetic-containing hexapeptide.,Phan J, Lee K, Cherry S, Tropea JE, Burke TR Jr, Waugh DS Biochemistry. 2003 Nov 18;42(45):13113-21. PMID:14609321<ref>PMID:14609321</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1qz0" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
Yersinia pestis, the causative agent of bubonic plague, secretes a eukaryotic-like protein tyrosine phosphatase (PTPase) termed Yersinia outer protein H (YopH) that is essential for virulence. We have determined, for the first time, the crystal structure of the YopH PTPase domain in complex with a nonhydrolyzable substrate analogue, the hexapeptide mimetic Ac-DADE-F(2)Pmp-L-NH(2). As anticipated, the mode of ligand binding in the active site is similar to the way in which the corresponding phosphohexapeptide binds to the structurally homologous human PTP1B. Unexpectedly, however, the crystal structure also revealed a second substrate-binding site in YopH that is not present in PTP1B. The mode of binding and structural conformation of the hexapeptide analogue is quite different in the two sites. Although the biological function of the second substrate-binding site remains to be investigated, the structure of a substrate analogue in the active site of Y. pestis YopH opens the door for the structure-based design and optimization of therapeutic countermeasures to combat this potential agent of bioterrorism.
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
 
== References ==
==About this Structure==
<references/>
1QZ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZ0 OCA].
__TOC__
 
</StructureSection>
==Reference==
[[Category: Large Structures]]
High-resolution structure of the Yersinia pestis protein tyrosine phosphatase YopH in complex with a phosphotyrosyl mimetic-containing hexapeptide., Phan J, Lee K, Cherry S, Tropea JE, Burke TR Jr, Waugh DS, Biochemistry. 2003 Nov 18;42(45):13113-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14609321 14609321]
[[Category: Protein-tyrosine-phosphatase]]
[[Category: Single protein]]
[[Category: Yersinia pestis]]
[[Category: Yersinia pestis]]
[[Category: Cherry, S.]]
[[Category: Burke Jr TR]]
[[Category: Jr., T R.Burke.]]
[[Category: Cherry S]]
[[Category: Lee, K.]]
[[Category: Lee K]]
[[Category: Phan, J.]]
[[Category: Phan J]]
[[Category: Tropea, J E.]]
[[Category: Tropea JE]]
[[Category: Waugh, D S.]]
[[Category: Waugh DS]]
[[Category: dephosphorylase]]
[[Category: hydrolase]]
[[Category: phosphatase]]
[[Category: ptpase]]
[[Category: yoph]]
 
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