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[[Image:2giz.png|left|200px]]


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==Structural and functional analysis of Natrin, a member of crisp-3 family blocks a variety of ion channels==
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<StructureSection load='2giz' size='340' side='right'caption='[[2giz]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2giz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2giz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2giz OCA], [https://pdbe.org/2giz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2giz RCSB], [https://www.ebi.ac.uk/pdbsum/2giz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2giz ProSAT]</span></td></tr>
{{STRUCTURE_2giz|  PDB=2giz  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRVP1_NAJAT CRVP1_NAJAT] Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.<ref>PMID:15581679</ref> <ref>PMID:17070778</ref> <ref>PMID:18658224</ref> <ref>PMID:16042391</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
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    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gi/2giz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2giz ConSurf].
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== Publication Abstract from PubMed ==
Cysteine-rich secretory proteins (CRISPs) are secreted single-chain proteins found in different sources. Natrin is a member of the CRISP family purified from the snake venom of Naja naja atra, which has been reported as a BKca channel blocker. In our study, crystals of natrin were obtained in two different crystal forms and the structure of one of them was solved at a resolution of 1.68A. Our electrophysiological experiments indicated that natrin can block the ion channel currents of the voltage-gated potassium channel Kv1.3. Docking analyses of the interaction between natrin and Kv1.3 revealed a novel interaction pattern different from the two previously reported K(+) channel inhibition models termed "functional dyad" and "basic ring". These findings offered new insights into the function of natrin and how the specific interactions between CRISPs and different ion channels can be achieved.


===Structural and functional analysis of Natrin, a member of crisp-3 family blocks a variety of ion channels===
Structural and functional analysis of natrin, a venom protein that targets various ion channels.,Wang F, Li H, Liu MN, Song H, Han HM, Wang QL, Yin CC, Zhou YC, Qi Z, Shu YY, Lin ZJ, Jiang T Biochem Biophys Res Commun. 2006 Dec 15;351(2):443-8. Epub 2006 Oct 20. PMID:17070778<ref>PMID:17070778</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 17070778 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_17070778}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2GIZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIZ OCA].
 
==Reference==
Structural and functional analysis of natrin, a venom protein that targets various ion channels., Wang F, Li H, Liu MN, Song H, Han HM, Wang QL, Yin CC, Zhou YC, Qi Z, Shu YY, Lin ZJ, Jiang T, Biochem Biophys Res Commun. 2006 Dec 15;351(2):443-8. Epub 2006 Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17070778 17070778]
[[Category: Naja atra]]
[[Category: Naja atra]]
[[Category: Single protein]]
[[Category: Jiang T]]
[[Category: Jiang, T.]]
[[Category: Li H]]
[[Category: Li, H.]]
[[Category: Lin Z]]
[[Category: Lin, Z.]]
[[Category: Qi Z]]
[[Category: Qi, Z.]]
[[Category: Shu Y]]
[[Category: Shu, Y.]]
[[Category: Wang F]]
[[Category: Wang, F.]]
[[Category: Yin C]]
[[Category: Yin, C.]]
[[Category: Zhou Y]]
[[Category: Zhou, Y.]]
[[Category: Blocker]]
[[Category: Crisp]]
[[Category: Crystal structure]]
[[Category: Docking]]
[[Category: Electrophysiology]]
 
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