2f9g: Difference between revisions

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-{{Seed}}
-[[Image:2f9g.png|left|200px]]
-<!--
+==Crystal structure of Fus3 phosphorylated on Tyr182==
-The line below this paragraph, containing "STRUCTURE_2f9g", creates the "Structure Box" on the page.
+<StructureSection load='2f9g' size='340' side='right'caption='[[2f9g]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2f9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F9G FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
-{{STRUCTURE_2f9g|  PDB=2f9g  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f9g OCA], [https://pdbe.org/2f9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f9g RCSB], [https://www.ebi.ac.uk/pdbsum/2f9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f9g ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FUS3_YEAST FUS3_YEAST] Together with closely related KSS1, FUS3 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated FUS3 activates the mating but suppresses the filamentation pathway, whereas activated KSS1 activates both pathways. Pheromone-activated FUS3 functions by inhibiting the binding of the transcriptional activator STE12 to filamentation specific genes while inducing its binding to and activity at mating specific genes. Non-activated FUS3 has a repressive effect on STE12 transcriptional activity. KSS1 can partially compensate for the lack of FUS3 but mating efficiency is reduced and the filamentation program is partially activated upon pheromone signaling. FUS3 phosphorylates STE7, STE5, FAR1, DIG1, DIG2 and STE12.<ref>PMID:9393860</ref> <ref>PMID:9094309</ref> <ref>PMID:11583629</ref> <ref>PMID:12732146</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f9/2f9g_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f9g ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.
-===Crystal structure of Fus3 phosphorylated on Tyr182===
+The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway.,Bhattacharyya RP, Remenyi A, Good MC, Bashor CJ, Falick AM, Lim WA Science. 2006 Feb 10;311(5762):822-6. Epub 2006 Jan 19. PMID:16424299<ref>PMID:16424299</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2f9g" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16424299}}, adds the Publication Abstract to the page
+*[[Mitogen-activated protein kinase 3D structures|Mitogen-activated protein kinase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16424299 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16424299}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-F9G is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9G OCA].
-==Reference==
-<ref group="xtra">PMID:16424299</ref><references group="xtra"/>
-[[Category: Non-specific serine/threonine protein kinase]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Bashor, C J.]]
+[[Category: Bashor CJ]]
-[[Category: Bhattacharyya, R P.]]
+[[Category: Bhattacharyya RP]]
-[[Category: Falick, A M.]]
+[[Category: Falick AM]]
-[[Category: Good, M C.]]
+[[Category: Good MC]]
-[[Category: Lim, W A.]]
+[[Category: Lim WA]]
-[[Category: Remenyi, A.]]
+[[Category: Remenyi A]]
-[[Category: Map kinase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:42:02 2009''