2axc: Difference between revisions

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-{{Seed}}
-[[Image:2axc.png|left|200px]]
-<!--
+==Crystal structure of ColE7 translocation domain==
-The line below this paragraph, containing "STRUCTURE_2axc", creates the "Structure Box" on the page.
+<StructureSection load='2axc' size='340' side='right'caption='[[2axc]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2axc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AXC FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_2axc|  PDB=2axc  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2axc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2axc OCA], [https://pdbe.org/2axc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2axc RCSB], [https://www.ebi.ac.uk/pdbsum/2axc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2axc ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CEA7_ECOLX CEA7_ECOLX] This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.  Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ax/2axc_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2axc ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+ColE7 is a nuclease-type colicin released from Escherichia coli to kill sensitive bacterial cells by degrading the nucleic acid molecules in their cytoplasm. ColE7 is classified as one of the group A colicins, since the N-terminal translocation domain (T-domain) of the nuclease-type colicins interact with specific membrane-bound or periplasmic Tol proteins during protein import. Here, we show that if the N-terminal tail of ColE7 is deleted, ColE7 (residues 63-576) loses its bactericidal activity against E.coli. Moreover, TolB protein interacts directly with the T-domain of ColE7 (residues 1-316), but not with the N-terminal deleted T-domain (residues 60-316), as detected by co-immunoprecipitation experiments, confirming that the N-terminal tail is required for ColE7 interactions with TolB. The crystal structure of the N-terminal tail deleted ColE7 T-domain was determined by the multi-wavelength anomalous dispersion method at a resolution of 1.7 angstroms. The structure of the ColE7 T-domain superimposes well with the T-domain of ColE3 and TR-domain of ColB, a group A Tol-dependent colicin and a group B TonB-dependent colicin, respectively. The structural resemblance of group A and B colicins implies that the two groups of colicins may share a mechanistic connection during cellular import.
-===Crystal structure of ColE7 translocation domain===
+High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes.,Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS J Mol Biol. 2006 Feb 10;356(1):22-31. Epub 2005 Dec 5. PMID:16360169<ref>PMID:16360169</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2axc" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16360169}}, adds the Publication Abstract to the page
+*[[Colicin 3D structures|Colicin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16360169 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16360169}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Escherichia coli str. K-12 substr. W3110]]
-AXC is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXC OCA].
+[[Category: Large Structures]]
+[[Category: Chak KF]]
-==Reference==
+[[Category: Cheng YS]]
-<ref group="xtra">PMID:16360169</ref><references group="xtra"/>
+[[Category: Doudeva LG]]
-[[Category: Escherichia coli]]
+[[Category: Shi Z]]
-[[Category: Chak, K F.]]
+[[Category: Yang WZ]]
-[[Category: Cheng, Y S.]]
+[[Category: Yuan HS]]
-[[Category: Doudeva, L G.]]
-[[Category: Shi, Z.]]
-[[Category: Yang, W Z.]]
-[[Category: Yuan, H S.]]
-[[Category: Colicin]]
-[[Category: Translocation domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 07:50:39 2009''