2ab5: Difference between revisions

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-[[Image:2ab5.png|left|200px]]
-{{STRUCTURE_2ab5|  PDB=2ab5  |  SCENE=  }}
+==bI3 LAGLIDADG Maturase==
+<StructureSection load='2ab5' size='340' side='right'caption='[[2ab5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ab5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AB5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ab5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab5 OCA], [https://pdbe.org/2ab5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ab5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ab5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MBI3_YEAST MBI3_YEAST] Mitochondrial mRNA maturase required for splicing of intron 3 of the cytochrome b (COB) gene, containing its own coding sequence. In vivo splicing requires the formation of a ribonucleoprotein complex together with the imported mitochondrial RNA-splicing protein MRS1. The complex seems to stimulate the intrinsic ribozyme activity of intron bI3 through binding to and stabilizing specific secondary and tertiary structure elements in the RNA.<ref>PMID:11773622</ref> <ref>PMID:12924947</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/2ab5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ab5 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+LAGLIDADG endonucleases bind across adjacent major grooves via a saddle-shaped surface and catalyze DNA cleavage. Some LAGLIDADG proteins, called maturases, facilitate splicing by group I introns, raising the issue of how a DNA-binding protein and an RNA have evolved to function together. In this report, crystallographic analysis shows that the global architecture of the bI3 maturase is unchanged from its DNA-binding homologs; in contrast, the endonuclease active site, dispensable for splicing facilitation, is efficiently compromised by a lysine residue replacing essential catalytic groups. Biochemical experiments show that the maturase binds a peripheral RNA domain 50 A from the splicing active site, exemplifying long-distance structural communication in a ribonucleoprotein complex. The bI3 maturase nucleic acid recognition saddle interacts at the RNA minor groove; thus, evolution from DNA to RNA function has been mediated by a switch from major to minor groove interaction.
-===bI3 LAGLIDADG Maturase===
+Evolution from DNA to RNA recognition by the bI3 LAGLIDADG maturase.,Longo A, Leonard CW, Bassi GS, Berndt D, Krahn JM, Hall TM, Weeks KM Nat Struct Mol Biol. 2005 Sep;12(9):779-87. Epub 2005 Aug 21. PMID:16116439<ref>PMID:16116439</ref>
-{{ABSTRACT_PUBMED_16116439}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2ab5" style="background-color:#fffaf0;"></div>
-[[2ab5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB5 OCA].
 ==See Also==
 *[[Non-Standard Residue|Non-Standard Residue]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:016116439</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Bassi, G S.]]
+[[Category: Bassi GS]]
-[[Category: Berndt, D.]]
+[[Category: Berndt D]]
-[[Category: Hall, T M.]]
+[[Category: Hall TM]]
-[[Category: Krahn, J M.]]
+[[Category: Krahn JM]]
-[[Category: Leonard, C W.]]
+[[Category: Leonard CW]]
-[[Category: Longo, A.]]
+[[Category: Longo A]]
-[[Category: Weeks, K M.]]
+[[Category: Weeks KM]]
-[[Category: Group i intron splicing]]
-[[Category: Laglidadg endonuclease]]
-[[Category: Maturase]]
-[[Category: Protein binding]]
-[[Category: Rna binding]]