1yz2: Difference between revisions
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==Solution structure of Am2766== | ==Solution structure of Am2766== | ||
<StructureSection load='1yz2' size='340' side='right' caption='[[1yz2 | <StructureSection load='1yz2' size='340' side='right'caption='[[1yz2]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1yz2]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1yz2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_amadis Conus amadis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YZ2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yz2 OCA], [https://pdbe.org/1yz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yz2 RCSB], [https://www.ebi.ac.uk/pdbsum/1yz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yz2 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/O166_CONAA O166_CONAA] Delta-conotoxins bind to site 6 of voltage-gated sodium channels (Nav) and inhibit the inactivation process.<ref>PMID:14550575</ref> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The three-dimensional (3D) NMR solution structure (MeOH) of the highly hydrophobic delta-conotoxin delta-Am2766 from the molluscivorous snail Conus amadis has been determined. Fifteen converged structures were obtained on the basis of 262 distance constraints, 25 torsion-angle constraints, and ten constraints based on disulfide linkages and H-bonds. The root-mean-square deviations (rmsd) about the averaged coordinates of the backbone (N, C(alpha), C) and (all) heavy atoms were 0.62+/-0.20 and 1.12+/-0.23 A, respectively. The structures determined are of good stereochemical quality, as evidenced by the high percentage (100%) of backbone dihedral angles that occupy favorable and additionally allowed regions of the Ramachandran map. The structure of delta-Am2766 consists of a triple-stranded antiparallel beta-sheet, and of four turns. The three disulfides form the classical 'inhibitory cysteine knot' motif. So far, only one tertiary structure of a delta-conotoxin has been reported; thus, the tertiary structure of delta-Am2766 is the second such example. Another Conus peptide, Am2735 from C. amadis, has also been purified and sequenced. Am2735 shares 96% sequence identity with delta-Am2766. Unlike delta-Am2766, Am2735 does not inhibit the fast inactivation of Na+ currents in rat brain Na(v)1.2 Na+ channels at concentrations up to 200 nM. | |||
Solution structure of delta-Am2766: a highly hydrophobic delta-conotoxin from Conus amadis that inhibits inactivation of neuronal voltage-gated sodium channels.,Sarma SP, Kumar GS, Sudarslal S, Iengar P, Ramasamy P, Sikdar SK, Krishnan KS, Balaram P Chem Biodivers. 2005 Apr;2(4):535-56. doi: 10.1002/cbdv.200590035. PMID:17192003<ref>PMID:17192003</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1yz2" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Conus amadis]] | [[Category: Conus amadis]] | ||
[[Category: Balaram | [[Category: Large Structures]] | ||
[[Category: Iengar | [[Category: Balaram P]] | ||
[[Category: Krishnan | [[Category: Iengar P]] | ||
[[Category: Kumar | [[Category: Krishnan KS]] | ||
[[Category: Sarma | [[Category: Kumar GS]] | ||
[[Category: Sikdar | [[Category: Sarma SP]] | ||
[[Category: Sudarslal | [[Category: Sikdar SK]] | ||
[[Category: Sudarslal S]] | |||
Latest revision as of 03:43, 21 November 2024
Solution structure of Am2766Solution structure of Am2766
Structural highlights
FunctionO166_CONAA Delta-conotoxins bind to site 6 of voltage-gated sodium channels (Nav) and inhibit the inactivation process.[1] Publication Abstract from PubMedThe three-dimensional (3D) NMR solution structure (MeOH) of the highly hydrophobic delta-conotoxin delta-Am2766 from the molluscivorous snail Conus amadis has been determined. Fifteen converged structures were obtained on the basis of 262 distance constraints, 25 torsion-angle constraints, and ten constraints based on disulfide linkages and H-bonds. The root-mean-square deviations (rmsd) about the averaged coordinates of the backbone (N, C(alpha), C) and (all) heavy atoms were 0.62+/-0.20 and 1.12+/-0.23 A, respectively. The structures determined are of good stereochemical quality, as evidenced by the high percentage (100%) of backbone dihedral angles that occupy favorable and additionally allowed regions of the Ramachandran map. The structure of delta-Am2766 consists of a triple-stranded antiparallel beta-sheet, and of four turns. The three disulfides form the classical 'inhibitory cysteine knot' motif. So far, only one tertiary structure of a delta-conotoxin has been reported; thus, the tertiary structure of delta-Am2766 is the second such example. Another Conus peptide, Am2735 from C. amadis, has also been purified and sequenced. Am2735 shares 96% sequence identity with delta-Am2766. Unlike delta-Am2766, Am2735 does not inhibit the fast inactivation of Na+ currents in rat brain Na(v)1.2 Na+ channels at concentrations up to 200 nM. Solution structure of delta-Am2766: a highly hydrophobic delta-conotoxin from Conus amadis that inhibits inactivation of neuronal voltage-gated sodium channels.,Sarma SP, Kumar GS, Sudarslal S, Iengar P, Ramasamy P, Sikdar SK, Krishnan KS, Balaram P Chem Biodivers. 2005 Apr;2(4):535-56. doi: 10.1002/cbdv.200590035. PMID:17192003[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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