1tg1: Difference between revisions

==Crystal Structure of the complex formed between russells viper phospholipase A2 and a designed peptide inhibitor PHQ-Leu-Val-Arg-Tyr at 1.2A resolution==

<StructureSection load='1tg1' size='340' side='right'caption='[[1tg1]], [[Resolution|resolution]] 1.25&Aring;' scene=''>

== Structural highlights ==

<table><tr><td colspan='2'>[[1tg1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Daboia_russelii_russelii Daboia russelii russelii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TG1 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene>, <scene name='pdbligand=PHQ:BENZYL+CHLOROCARBONATE'>PHQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tg1 OCA], [https://pdbe.org/1tg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tg1 RCSB], [https://www.ebi.ac.uk/pdbsum/1tg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tg1 ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/PA2B8_DABRR PA2B8_DABRR] Snake venom phospholipase A2 (PLA2) that shows weak neurotoxicity and medium anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC(50) is 130 nM) (PubMed:18062812). It also damages vital organs such as lung, liver and kidney, displays edema-inducing activities when injected into the foot pads of mice and induces necrosis of muscle cells when injected into the thigh muscle. Has a low enzymatic activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:18062812</ref> <ref>PMID:2115497</ref> <ref>PMID:8835338</ref>

[[Image:Consurf_key_small.gif|200px|right]]

    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tg1_consurf.spt"</scriptWhenChecked>

    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>

    <text>to colour the structure by Evolutionary Conservation</text>

  </jmolCheckbox>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tg1 ConSurf].

<div style="clear:both"></div>

==See Also==

*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]

<references/>

</StructureSection>

[[Category: Daboia russelii russelii]]

[[Category: Large Structures]]

[[Category: Synthetic construct]]

[[Category: Betzel C]]

[[Category: Dey S]]

[[Category: Kaur P]]

[[Category: Perbandt M]]

[[Category: Sharma S]]

[[Category: Singh N]]

[[Category: Singh TP]]

[[Category: Somvanshi RK]]