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New page: left|200px<br /><applet load="1srb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1srb" /> '''CONFORMATIONAL STUDIES ON SRTB, A NON-SELECT... |
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== | ==CONFORMATIONAL STUDIES ON SRTB, A NON-SELECTIVE ENDOTHELIN RECEPTOR AGONIST, AND ON IRL 1620, AN ETB RECEPTOR SPECIFIC AGONIST== | ||
1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin | <StructureSection load='1srb' size='340' side='right'caption='[[1srb]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1srb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atractaspis_andersonii Atractaspis andersonii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SRB FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1srb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1srb OCA], [https://pdbe.org/1srb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1srb RCSB], [https://www.ebi.ac.uk/pdbsum/1srb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1srb ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SRTX_ATREN SRTX_ATREN] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.<ref>PMID:21889567</ref> Sarafotoxin-B: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities) (PubMed:21889567).<ref>PMID:21889567</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin SRTb have identified a helix between residues Asp 8 and His 16, and a beta-turn involving residues Cys 3 to Met 6; however, the biologically important C-terminal five residues were found to be conformationally variable. The average RMSD, measured for the final 43 refined structures to the average structure over residues 1-16, was 0.78 +/- 0.18 A for the backbone atoms and 1.39 +/- 0.22 A for all atoms. The torsion angles Cys 3 psi/Lys 4 theta, Thr 7 psi/Asp 8 theta and Gln 17 theta were identified as sites of conformational variability. Differences were found between the structures in the bicyclic loop region for SRTb and those published for ET1, another nonselective receptor agonist, which may explain the observed differences in potency of these peptides. The conformation of an ETB receptor-specific agonist, IRL 1620, which lacks the N-terminal seven residues and the two intrachain disulfides, was found by NMR and circular dichroism spectroscopy to be predominantly random coil, despite the fact that its affinity for the ETB receptor almost equals that of ET1. However, close analysis of the NMR results indicated the presence of turn-like structures, or a nascent helix, in the part of the sequence corresponding to the helical region in the parent peptides. These results suggest that the helical conformation may be required for ligand binding to the ETB receptor as well as to the ETA receptor. | |||
1H NMR studies of sarafotoxin SRTb, a nonselective endothelin receptor agonist, and IRL 1620, an ETB receptor-specific agonist.,Atkins AR, Martin RC, Smith R Biochemistry. 1995 Feb 14;34(6):2026-33. PMID:7849060<ref>PMID:7849060</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: Atractaspis | <div class="pdbe-citations 1srb" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Atkins | <references/> | ||
[[Category: Smith | __TOC__ | ||
</StructureSection> | |||
[[Category: Atractaspis andersonii]] | |||
[[Category: Large Structures]] | |||
[[Category: Atkins AR]] | |||
[[Category: Smith R]] | |||
Latest revision as of 10:36, 23 October 2024
CONFORMATIONAL STUDIES ON SRTB, A NON-SELECTIVE ENDOTHELIN RECEPTOR AGONIST, AND ON IRL 1620, AN ETB RECEPTOR SPECIFIC AGONISTCONFORMATIONAL STUDIES ON SRTB, A NON-SELECTIVE ENDOTHELIN RECEPTOR AGONIST, AND ON IRL 1620, AN ETB RECEPTOR SPECIFIC AGONIST
Structural highlights
FunctionSRTX_ATREN Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.[1] Sarafotoxin-B: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities) (PubMed:21889567).[2] Publication Abstract from PubMed1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin SRTb have identified a helix between residues Asp 8 and His 16, and a beta-turn involving residues Cys 3 to Met 6; however, the biologically important C-terminal five residues were found to be conformationally variable. The average RMSD, measured for the final 43 refined structures to the average structure over residues 1-16, was 0.78 +/- 0.18 A for the backbone atoms and 1.39 +/- 0.22 A for all atoms. The torsion angles Cys 3 psi/Lys 4 theta, Thr 7 psi/Asp 8 theta and Gln 17 theta were identified as sites of conformational variability. Differences were found between the structures in the bicyclic loop region for SRTb and those published for ET1, another nonselective receptor agonist, which may explain the observed differences in potency of these peptides. The conformation of an ETB receptor-specific agonist, IRL 1620, which lacks the N-terminal seven residues and the two intrachain disulfides, was found by NMR and circular dichroism spectroscopy to be predominantly random coil, despite the fact that its affinity for the ETB receptor almost equals that of ET1. However, close analysis of the NMR results indicated the presence of turn-like structures, or a nascent helix, in the part of the sequence corresponding to the helical region in the parent peptides. These results suggest that the helical conformation may be required for ligand binding to the ETB receptor as well as to the ETA receptor. 1H NMR studies of sarafotoxin SRTb, a nonselective endothelin receptor agonist, and IRL 1620, an ETB receptor-specific agonist.,Atkins AR, Martin RC, Smith R Biochemistry. 1995 Feb 14;34(6):2026-33. PMID:7849060[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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