1s7z: Difference between revisions

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OCA

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-[[Image:1s7z.jpg|left|200px]]<br /><applet load="1s7z" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1s7z, resolution 1.83&Aring;" />
-'''Structure of Ocr from Bacteriophage T7'''<br />
-==Overview==
+==Structure of Ocr from Bacteriophage T7==
-We have solved, by X-ray crystallography to a resolution of 1.8 A, the, structure of a protein capable of mimicking approximately 20 base pairs of, B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of, negative charges along the phosphate backbone of B-form DNA. We also, demonstrate that ocr is an efficient inhibitor in vivo of all known, families of the complex type I DNA restriction enzymes. Using atomic force, microscopy, we have also observed that type I enzymes induce a bend in DNA, of similar magnitude to the bend in the ocr molecule. This first structure, of an antirestriction protein demonstrates the construction of structural, mimetics of long segments of B-form DNA.
+<StructureSection load='1s7z' size='340' side='right'caption='[[1s7z]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1s7z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S7Z FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7z OCA], [https://pdbe.org/1s7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s7z RCSB], [https://www.ebi.ac.uk/pdbsum/1s7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7z ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/OCR_BPT7 OCR_BPT7]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s7/1s7z_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s7z ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have solved, by X-ray crystallography to a resolution of 1.8 A, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA.
-==About this Structure==
+Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA.,Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597<ref>PMID:11804597</ref>
-S7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with CS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11804597 11804597]
+</div>
-[[Category: Bacteriophage t7]]
+<div class="pdbe-citations 1s7z" style="background-color:#fffaf0;"></div>
-[[Category: Single protein]]
+== References ==
-[[Category: Atanasiu, C.]]
+<references/>
-[[Category: Berg, T.]]
+__TOC__
-[[Category: Dryden, D.T.]]
+</StructureSection>
-[[Category: Edwardson, J.M.]]
+[[Category: Escherichia phage T7]]
-[[Category: Henderson, R.M.]]
+[[Category: Large Structures]]
-[[Category: Sturrock, S.S.]]
+[[Category: Atanasiu C]]
-[[Category: Taylor, P.]]
+[[Category: Berg T]]
-[[Category: Walkinshaw, M.D.]]
+[[Category: Dryden DT]]
-[[Category: CS]]
+[[Category: Edwardson JM]]
-[[Category: all helical]]
+[[Category: Henderson RM]]
-[[Category: gene regulation]]
+[[Category: Sturrock SS]]
+[[Category: Taylor P]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:11:16 2007''
+[[Category: Walkinshaw MD]]