1pvb: Difference between revisions

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-[[Image:1pvb.jpg|left|200px]]<br /><applet load="1pvb" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1pvb, resolution 1.75&Aring;" />
-'''X-RAY STRUCTURE OF A NEW CRYSTAL FORM OF PIKE 4.10 PARVALBUMIN'''<br />
-==Overview==
+==X-RAY STRUCTURE OF A NEW CRYSTAL FORM OF PIKE 4.10 PARVALBUMIN==
-A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in, presence of EDTA at pH 8.0. The crystals are orthorhombic, space group, P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data, were collected to 1.75 A. The structure was solved by molecular, replacement and refined to R = 0.168 for 7774 observed reflections [I&gt;/=, 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the, previously reported structures, the main differences concern the, conformation of the N-terminal residues and the packing in the unit cell.
+<StructureSection load='1pvb' size='340' side='right'caption='[[1pvb]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1pvb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PVB FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pvb OCA], [https://pdbe.org/1pvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pvb RCSB], [https://www.ebi.ac.uk/pdbsum/1pvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pvb ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PRVB_ESOLU PRVB_ESOLU] In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pv/1pvb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pvb ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in presence of EDTA at pH 8.0. The crystals are orthorhombic, space group P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data were collected to 1.75 A. The structure was solved by molecular replacement and refined to R = 0.168 for 7774 observed reflections [I&gt;/= 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the previously reported structures, the main differences concern the conformation of the N-terminal residues and the packing in the unit cell.
-==About this Structure==
+X-ray structure of a new crystal form of pike 4.10 beta parvalbumin.,Declercq JP, Tinant B, Parello J Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):165-9. PMID:15299738<ref>PMID:15299738</ref>
-PVB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius] with CA, NH4 and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PVB OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-X-ray structure of a new crystal form of pike 4.10 beta parvalbumin., Declercq JP, Tinant B, Parello J, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):165-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299738 15299738]
+</div>
+<div class="pdbe-citations 1pvb" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Parvalbumin|Parvalbumin]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Esox lucius]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Declercq, J.P.]]
+[[Category: Declercq JP]]
-[[Category: Parello, J.]]
+[[Category: Parello J]]
-[[Category: Tinant, B.]]
+[[Category: Tinant B]]
-[[Category: ACE]]
-[[Category: CA]]
-[[Category: NH4]]
-[[Category: calcium binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:09:10 2007''