1ps5: Difference between revisions

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-[[Image:1ps5.jpg|left|200px]]<br /><applet load="1ps5" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1ps5, resolution 2.00&Aring;" />
-'''STRUCTURE OF THE MONOCLINIC C2 FORM OF HEN EGG-WHITE LYSOZYME AT 2.0 ANGSTROMS RESOLUTION'''<br />
-==Overview==
+==STRUCTURE OF THE MONOCLINIC C2 FORM OF HEN EGG-WHITE LYSOZYME AT 2.0 ANGSTROMS RESOLUTION==
-Suitable conditions for protein crystallization are commonly identified by, screening combinations of independent factors that affect crystal, formation. Because precipitating agents are prime determinants of, crystallization, we investigated whether a systematic exploration of, combinations of mechanistically distinct precipitants would enhance, crystallization. A crystallization screen containing 64 precipitant, mixtures was devised. Tests with ten HIV envelope-related proteins, demonstrated that use of precipitant mixtures significantly enhanced both, the probability of crystallization as well as the quality of optimized, crystals. Tests with hen egg white lysozyme generated a novel C2 crystal, from a salt/organic solvent mixture; structure solution at 2 A resolution, revealed a lattice held together by both hydrophobic and electrostatic, dyad interactions. The results indicate that mechanistically distinct, precipitants can synergize, with precipitant combinations adding unique, dimensions to protein crystallization.
+<StructureSection load='1ps5' size='340' side='right'caption='[[1ps5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ps5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PS5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ps5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ps5 OCA], [https://pdbe.org/1ps5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ps5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ps5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ps5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/1ps5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ps5 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Suitable conditions for protein crystallization are commonly identified by screening combinations of independent factors that affect crystal formation. Because precipitating agents are prime determinants of crystallization, we investigated whether a systematic exploration of combinations of mechanistically distinct precipitants would enhance crystallization. A crystallization screen containing 64 precipitant mixtures was devised. Tests with ten HIV envelope-related proteins demonstrated that use of precipitant mixtures significantly enhanced both the probability of crystallization as well as the quality of optimized crystals. Tests with hen egg white lysozyme generated a novel C2 crystal from a salt/organic solvent mixture; structure solution at 2 A resolution revealed a lattice held together by both hydrophobic and electrostatic dyad interactions. The results indicate that mechanistically distinct precipitants can synergize, with precipitant combinations adding unique dimensions to protein crystallization.
-==About this Structure==
+Enhancing protein crystallization through precipitant synergy.,Majeed S, Ofek G, Belachew A, Huang CC, Zhou T, Kwong PD Structure. 2003 Sep;11(9):1061-70. PMID:12962625<ref>PMID:12962625</ref>
-PS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PS5 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Enhancing protein crystallization through precipitant synergy., Majeed S, Ofek G, Belachew A, Huang CC, Zhou T, Kwong PD, Structure. 2003 Sep;11(9):1061-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12962625 12962625]
+</div>
+<div class="pdbe-citations 1ps5" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Gallus gallus]]
-[[Category: Lysozyme]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Belachew A]]
-[[Category: Belachew, A.]]
+[[Category: Huang C]]
-[[Category: Huang, C.]]
+[[Category: Kwong PD]]
-[[Category: Kwong, P.D.]]
+[[Category: Majeed S]]
-[[Category: Majeed, S.]]
+[[Category: Ofek G]]
-[[Category: Ofek, G.]]
+[[Category: Zhou T]]
-[[Category: Zhou, T.]]
-[[Category: SO4]]
-[[Category: glycosidase]]
-[[Category: hydrolase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:04:31 2007''