1ntn: Difference between revisions

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-{{Seed}}
-[[Image:1ntn.png|left|200px]]
-<!--
+==THE CRYSTAL STRUCTURE OF NEUROTOXIN-I FROM NAJA NAJA OXIANA AT 1.9 ANGSTROMS RESOLUTION==
-The line below this paragraph, containing "STRUCTURE_1ntn", creates the "Structure Box" on the page.
+<StructureSection load='1ntn' size='340' side='right'caption='[[1ntn]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1ntn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_oxiana Naja oxiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NTN FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ntn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ntn OCA], [https://pdbe.org/1ntn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ntn RCSB], [https://www.ebi.ac.uk/pdbsum/1ntn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ntn ProSAT]</span></td></tr>
-{{STRUCTURE_1ntn|  PDB=1ntn  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/3L21_NAJOX 3L21_NAJOX] Binds with high affinity to muscular (tested on Torpedo marmorata AChR, Kd=0.38 nM) and neuronal (tested on chimeric alpha-7/CHRNA7, Kd=12 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). The toxin also shows a very weak inhibition of GABA(A) receptors composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) subunits (PubMed:26221036).<ref>PMID:26221036</ref> <ref>PMID:9305882</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1ntn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ntn ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Neurotoxin-1 from Naja naja oxiana venom (NTX-1) has been crystallized by vapor diffusion in sitting drops. The crystals have cell dimensions of a = 25.2 A, b = 75.6 A, c = 35.9 A, and are in space group P2(1)2(1)2(1). Three-dimensional data to 1.9 A have been recorded by a Syntex P2(1) automatic diffractometer. The atomic structure of the toxin has been determined by molecular replacement using the alpha-cobratoxin (alpha-CTX) as the search model. The position of 534 non-hydrogen protein atoms have been determined. The model contains 65 water molecules. Refinement has led to an R-factor of 19.3% at 1.9 A resolution. The secondary and tertiary structures of NTX-1 have been analyzed and a comparison with structure of the alpha-CTX has been made.
-===THE CRYSTAL STRUCTURE OF NEUROTOXIN-I FROM NAJA NAJA OXIANA AT 1.9 ANGSTROMS RESOLUTION===
+Three-dimensional structure of neurotoxin-1 from Naja naja oxiana venom at 1.9 A resolution.,Nickitenko AV, Michailov AM, Betzel C, Wilson KS FEBS Lett. 1993 Apr 5;320(2):111-7. PMID:8458425<ref>PMID:8458425</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_8458425}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1ntn" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 8458425 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_8458425}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-NTN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_oxiana Naja oxiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTN OCA].
-==Reference==
-Three-dimensional structure of neurotoxin-1 from Naja naja oxiana venom at 1.9 A resolution., Nickitenko AV, Michailov AM, Betzel C, Wilson KS, FEBS Lett. 1993 Apr 5;320(2):111-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8458425 8458425]
 [[Category: Naja oxiana]]
-[[Category: Single protein]]
+[[Category: Betzel C]]
-[[Category: Betzel, C.]]
+[[Category: Mikhailov AM]]
-[[Category: Mikhailov, A M.]]
+[[Category: Nickitenko AV]]
-[[Category: Nickitenko, A V.]]
+[[Category: Vainshtein BK]]
-[[Category: Vainshtein, B K.]]
+[[Category: Wilson K]]
-[[Category: Wilson, K.]]
-[[Category: Postsynaptic neurotoxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:22:36 2008''