1mm0: Difference between revisions
No edit summary |
No edit summary |
||
| (12 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
< | ==Solution structure of termicin, an antimicrobial peptide from the termite Pseudacanthotermes spiniger== | ||
<StructureSection load='1mm0' size='340' side='right'caption='[[1mm0]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1mm0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudacanthotermes_spiniger Pseudacanthotermes spiniger]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MM0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | |||
-- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm0 OCA], [https://pdbe.org/1mm0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mm0 RCSB], [https://www.ebi.ac.uk/pdbsum/1mm0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm0 ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TERN_PSEUS TERN_PSEUS] Weak activity against Gram-positive bacteria B.megaterium, S.pyogenes and M.luteus, strong activity against yeasts C.albicans, C.neoformans and S.cerevisiae and filamentous fungi F.oxysporum, F.culmorum, N.crassa and N.hematococca. Less active against filamentous fungus T.viride. Inactive against Gram-positive bacteria A.viridans and S.aureus, filamentous fungi A.fumigatus and B.bassiana and yeast C.glabrata. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
== | Check<jmol> | ||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/1mm0_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mm0 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The solution structure of termicin from hemocytes of the termite Pseudacanthotermes spiniger was determined by proton two-dimensional nuclear magnetic resonance spectroscopy and molecular modeling techniques. Termicin is a cysteine-rich antifungal peptide also exhibiting a weak antibacterial activity. The global fold of termicin consists of an alpha-helical segment (Phe4-Gln14) and a two-stranded (Phe19-Asp25 and Gln28-Phe33) antiparallel beta-sheet forming a "cysteine stabilized alphabeta motif" (CSalphabeta) also found in antibacterial and antifungal defensins from insects and from plants. Interestingly, termicin shares more structural similarities with the antibacterial insect defensins and with MGD-1, a mussel defensin, than with the insect antifungal defensins such as drosomycin and heliomicin. These structural comparisons suggest that global fold alone does not explain the difference between antifungals and antibacterials. The antifungal properties of termicin may be related to its marked hydrophobicity and its amphipatic structure as compared to the antibacterial defensins. [SWISS-PROT accession number: Termicin (P82321); PDB accession number: 1MM0.] | The solution structure of termicin from hemocytes of the termite Pseudacanthotermes spiniger was determined by proton two-dimensional nuclear magnetic resonance spectroscopy and molecular modeling techniques. Termicin is a cysteine-rich antifungal peptide also exhibiting a weak antibacterial activity. The global fold of termicin consists of an alpha-helical segment (Phe4-Gln14) and a two-stranded (Phe19-Asp25 and Gln28-Phe33) antiparallel beta-sheet forming a "cysteine stabilized alphabeta motif" (CSalphabeta) also found in antibacterial and antifungal defensins from insects and from plants. Interestingly, termicin shares more structural similarities with the antibacterial insect defensins and with MGD-1, a mussel defensin, than with the insect antifungal defensins such as drosomycin and heliomicin. These structural comparisons suggest that global fold alone does not explain the difference between antifungals and antibacterials. The antifungal properties of termicin may be related to its marked hydrophobicity and its amphipatic structure as compared to the antibacterial defensins. [SWISS-PROT accession number: Termicin (P82321); PDB accession number: 1MM0.] | ||
Solution structure of termicin, an antimicrobial peptide from the termite Pseudacanthotermes spiniger.,Da Silva P, Jouvensal L, Lamberty M, Bulet P, Caille A, Vovelle F Protein Sci. 2003 Mar;12(3):438-46. PMID:12592014<ref>PMID:12592014</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1mm0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudacanthotermes spiniger]] | [[Category: Pseudacanthotermes spiniger]] | ||
[[Category: | [[Category: Bulet P]] | ||
[[Category: | [[Category: Caille A]] | ||
[[Category: | [[Category: Da Silva P]] | ||
[[Category: Jouvensal | [[Category: Jouvensal L]] | ||
[[Category: Lamberty | [[Category: Lamberty M]] | ||
[[Category: Vovelle F]] | |||
[[Category: Vovelle | |||