1kwo: Difference between revisions

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-{{Seed}}
-[[Image:1kwo.png|left|200px]]
-<!--
+==SCALLOP MYOSIN S1-ATPgammaS-p-PDM IN THE ACTIN-DETACHED CONFORMATION==
-The line below this paragraph, containing "STRUCTURE_1kwo", creates the "Structure Box" on the page.
+<StructureSection load='1kwo' size='340' side='right'caption='[[1kwo]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1kwo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KWO FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PDM:4-[4-(2,5-DIOXO-PYRROLIDIN-1-YL)-PHENYLAMINO]-4-HYDROXY-BUTYRIC+ACID'>PDM</scene></td></tr>
-{{STRUCTURE_1kwo|  PDB=1kwo  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kwo OCA], [https://pdbe.org/1kwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kwo RCSB], [https://www.ebi.ac.uk/pdbsum/1kwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kwo ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MYS_ARGIR MYS_ARGIR] Muscle contraction.  Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kw/1kwo_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kwo ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Here we report a 2.3-A crystal structure of scallop myosin S1 complexed with ADP.BeF(x), as well as three additional structures (at 2.8-3.8 A resolution) for this S1 complexed with ATP analogs, some of which are cross-linked by para-phenyl dimaleimide, a short intramolecular cross-linker. In all cases, the complexes are characterized by an unwound SH1 helix first seen in an unusual 2.5-A scallop myosin-MgADP structure and described as corresponding to a previously unrecognized actin-detached internally uncoupled state. The unwinding of the SH1 helix effectively uncouples the converter/lever arm module from the motor and allows cross-linking by para-phenyl dimaleimide, which has been shown to occur only in weak actin-binding states of the molecule. Mutations near the metastable SH1 helix that disable the motor can be accounted for by viewing this structural element as a clutch controlling the transmission of torque to the lever arm. We have also determined a 3.2-A nucleotide-free structure of scallop myosin S1, which suggests that in the near-rigor state there are two conformations in the switch I loop, depending on whether nucleotide is present. Analysis of the subdomain motions in the weak actin-binding states revealed by x-ray crystallography, together with recent electron microscopic results, clarify the mechanical roles of the parts of the motor in the course of the contractile cycle and suggest how strong binding to actin triggers both the power stroke and product release.
-===SCALLOP MYOSIN S1-ATPgammaS-p-PDM IN THE ACTIN-DETACHED CONFORMATION===
+Crystallographic findings on the internally uncoupled and near-rigor states of myosin: further insights into the mechanics of the motor.,Himmel DM, Gourinath S, Reshetnikova L, Shen Y, Szent-Gyorgyi AG, Cohen C Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12645-50. Epub 2002 Sep 24. PMID:12297624<ref>PMID:12297624</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1kwo" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_12297624}}, adds the Publication Abstract to the page
+*[[Myosin 3D Structures|Myosin 3D Structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 12297624 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12297624}}
+__TOC__
+</StructureSection>
-==About this Structure==
-KWO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWO OCA].
-==Reference==
-Crystallographic findings on the internally uncoupled and near-rigor states of myosin: further insights into the mechanics of the motor., Himmel DM, Gourinath S, Reshetnikova L, Shen Y, Szent-Gyorgyi AG, Cohen C, Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12645-50. Epub 2002 Sep 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12297624 12297624]
 [[Category: Argopecten irradians]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Cohen, C.]]
+[[Category: Cohen C]]
-[[Category: Gourinath, S.]]
+[[Category: Gourinath S]]
-[[Category: Himmel, D M.]]
+[[Category: Himmel DM]]
-[[Category: Reshetnikova, L.]]
+[[Category: Reshetnikova L]]
-[[Category: Shen, Y.]]
+[[Category: Shen Y]]
-[[Category: Szent-Gyorgyi, G.]]
+[[Category: Szent-Gyorgyi G]]
-[[Category: Actin-detached]]
-[[Category: Cross linker]]
-[[Category: Mechanics of motor]]
-[[Category: Myosin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  2 11:10:11 2008''