1i26: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1i26.png|left|200px]]
-<!--
+==SOLUTION STRUCTURE OF PTU-1, A TOXIN FROM THE ASSASSIN BUGS PEIRATES TURPIS THAT BLOCKS THE VOLTAGE SENSITIVE CALCIUM CHANNEL N-TYPE==
-The line below this paragraph, containing "STRUCTURE_1i26", creates the "Structure Box" on the page.
+<StructureSection load='1i26' size='340' side='right'caption='[[1i26]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1i26]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Peirates_turpis Peirates turpis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I26 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 26 models</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i26 OCA], [https://pdbe.org/1i26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i26 RCSB], [https://www.ebi.ac.uk/pdbsum/1i26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i26 ProSAT]</span></td></tr>
-{{STRUCTURE_1i26|  PDB=1i26  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PLK1_PEITU PLK1_PEITU] Binds reversibly and blocks N-type voltage-gated calcium channels (Cav).[UniProtKB:P58608]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Ptu1 is a toxin from the assassin bug Peirates turpis which has been demonstrated to bind reversibly the N-type calcium channels and to have lower affinity than the omega-conotoxin MVIIA. We have determined the solution structure of Ptu1 by use of conventional two-dimensional NMR techniques followed by distance-geometry and molecular dynamics. The calculated structure of Ptu1 belongs to the inhibitory cystin knot structural family (ICK) that consists of a compact disulfide-bonded core from which four loops emerge. Analysis of the 25 converged solutions indicates that the molecular structure of Ptu1 contains a 2-stranded antiparallel beta-sheet (residues 24-27 and 31-34) as the only secondary structure. The loop 2 that has been described to be critical for the binding of the toxin on the channel is similar in Ptu1 and MVIIA. In this loop, the critical residue, Tyr13, in MVIIA is retrieved in Ptu1 as Phe13, but the presence of an acidic residue (Asp16) in Ptu1 could disturb the binding of Ptu1 on the channel and could explain the lower affinity of Ptu1 toward the N-type calcium channel compared to the one of MVIIA. Analysis of the electrostatic charge's repartition gives some insights about the importance of the basic residues, which could interact with acidic residues of the channel and then provide a stabilization of the toxin on the channel.
-===SOLUTION STRUCTURE OF PTU-1, A TOXIN FROM THE ASSASSIN BUGS PEIRATES TURPIS THAT BLOCKS THE VOLTAGE SENSITIVE CALCIUM CHANNEL N-TYPE===
+Solution structure of Ptu1, a toxin from the assassin bug Peirates turpis that blocks the voltage-sensitive calcium channel N-type.,Bernard C, Corzo G, Mosbah A, Nakajima T, Darbon H Biochemistry. 2001 Oct 30;40(43):12795-800. PMID:11669615<ref>PMID:11669615</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_11669615}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1i26" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 11669615 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_11669615}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-I26 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Peirates_turpis Peirates turpis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I26 OCA].
-==Reference==
-Solution structure of Ptu1, a toxin from the assassin bug Peirates turpis that blocks the voltage-sensitive calcium channel N-type., Bernard C, Corzo G, Mosbah A, Nakajima T, Darbon H, Biochemistry. 2001 Oct 30;40(43):12795-800. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11669615 11669615]
 [[Category: Peirates turpis]]
-[[Category: Single protein]]
+[[Category: Bernard C]]
-[[Category: Bernard, C.]]
+[[Category: Corzo G]]
-[[Category: Corzo, G.]]
+[[Category: Darbon H]]
-[[Category: Darbon, H.]]
+[[Category: Mosbah A]]
-[[Category: Mosbah, A.]]
+[[Category: Nakajima T]]
-[[Category: Nakajima, T.]]
-[[Category: Assassin bug]]
-[[Category: Ca channel]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 10:18:09 2008''